Product Info Summary
SKU: | A00174-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Oct4/POU5F1 Antibody
SKU/Catalog Number
A00174-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Oct4/POU5F1 Antibody catalog # A00174-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oct4/POU5F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00174-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Oct4/POU5F1 recombinant protein (Position: E26-A300).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00174-1 is reactive to POU5F1 in Human, Mouse, Rat
Applications
A00174-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
Background of POU5F1
Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. It is mapped to 6p21.33. This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
![A00174 1 POU5F1 primary antibodies FC testing 1 A00174 1 POU5F1 primary antibodies FC testing 1](https://www.bosterbio.com/media/catalog/product/A/0/A00174-1-POU5F1-primary-antibodies-FC-testing-1.jpg)
Click image to see more details
Figure 1. Flow Cytometry analysis of 293T cells using anti-POU5F1 antibody (A00174-1).
Overlay histogram showing 293T cells stained with A00174-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POU5F1 Antibody (A00174-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![A00174 1 POU5F1 primary antibodies FC testing 2 A00174 1 POU5F1 primary antibodies FC testing 2](https://www.bosterbio.com/media/catalog/product/A/0/A00174-1-POU5F1-primary-antibodies-FC-testing-2.jpg)
Click image to see more details
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-POU5F1 antibody (A00174-1).
Overlay histogram showing HepG2 cells stained with A00174-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POU5F1 Antibody (A00174-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For POU5F1 (Source: Uniprot.org, NCBI)
Gene Name
POU5F1
Full Name
POU domain, class 5, transcription factor 1
Weight
Superfamily
POU transcription factor family
Alternative Names
class 5, transcription factor 1; MGC22487; Oct-3; OCT3Oct4; Oct-4; Octamer-binding protein 3; otc-4; OTF3POU domain class 5, transcription factor 1; OTF4; POU class 5 homeobox 1; POU-type homeodomain-containing DNA-binding protein POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 POU class 5 homeobox 1 POU domain, class 5, transcription factor 1|POU domain transcription factor OCT4|POU-type homeodomain-containing DNA-binding protein|octamer-binding protein 3|octamer-binding protein 4|octamer-binding transcription factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POU5F1, check out the POU5F1 Infographic
![POU5F1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POU5F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Oct4/POU5F1 Antibody (A00174-1)
Hello CJ!
A00174-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis
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Customer Q&As
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1 Customer Q&As for Anti-Oct4/POU5F1 Antibody
Question
We are currently using anti-Oct4/POU5F1 antibody A00174-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
The anti-Oct4/POU5F1 antibody (A00174-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-05