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Product Info Summary
| SKU: | A00174 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-POU5F1 Antibody
SKU/Catalog Number
A00174
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-POU5F1 Antibody (Catalog # A00174). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
POU5F1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-POU5F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00174)
Host
Rabbit
Contents
POU5F1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
POU5F1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human POU5F1. The immunogen is located within amino acids 260 - 310 of POU5F1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of IRF3 are known to exist.
Reactive Species
A00174 is reactive to POU5F1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
38571 MW
Background of POU5F1
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00174 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 μgg/mL.
Protein Target Info & Infographic
Gene/Protein Information For POU5F1 (Source: Uniprot.org, NCBI)
Gene Name
POU5F1
Full Name
POU domain, class 5, transcription factor 1
Weight
38571 MW
Superfamily
POU transcription factor family
Alternative Names
OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, OCT3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, 41915 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 POU class 5 homeobox 1 POU domain, class 5, transcription factor 1|POU domain transcription factor OCT4|POU-type homeodomain-containing DNA-binding protein|octamer-binding protein 3|octamer-binding protein 4|octamer-binding transcription factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POU5F1, check out the POU5F1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POU5F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POU5F1 Antibody (A00174)
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