Anti-Nuclear Mitotic Apparatus Protein (NuMA) Monoclonal Antibody

Product Name

Anti-Nuclear Mitotic Apparatus Protein (NuMA) Monoclonal Antibody

View all NuMA Antibodies

SKU/Catalog Number

M02018

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Nuclear Mitotic Apparatus Protein (NuMA) Monoclonal Antibody (Catalog # M02018). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Nuclear Mitotic Apparatus Protein (NuMA) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02018)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

Clonality

Monoclonal

Clone Number

Clone: SPM300

Isotype

IgM, kappa

Immunogen

Colon carcinoma 174T cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02018 is reactive to NUMA1 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

238260 MW

Background of NuMA

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02018 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For NUMA1 (Source: Uniprot.org, NCBI)

Alternative Names

Nuclear mitotic apparatus protein 1 ;NuMA protein ;Nuclear matrix protein-22;NMP-22;SP-H antigen ;NUMA1 ;NMP22 , NUMA ; NUMA1 NMP-22, NUMA nuclear mitotic apparatus protein 1 nuclear mitotic apparatus protein 1|SP-H |centrophilin stabilizes mitotic spindle in mitotic cells|nuclear matrix protein-22|structural nuclear protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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