NUMA1 Antibodies

Nuclear mitotic apparatus protein 1 (NUMA1)

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:7769006, PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074). Functions to tether the minus ends of MTs at the spindle poles, which can be vital for the establishment and maintenance of the spindle poles (PubMed:12445386, PubMed:11956313).

Plays a role in the creation of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(I) alpha proteins, that regulates the recruitment and anchorage of this dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and thus regulates the appropriate oritentation of the mitotic spindle (PubMed:23027904, PubMed:22327364, PubMed:23921553).

Gene Information

Human
Gene Name:	NUMA1
Uniprot:	Q14980
Entrez:	4926

Family

Belongs to:
No superfamily

Alternative Names

centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; nuclear mitotic apparatus protein 1; NuMA protein; NuMA; NUMA1; SP-H Antigen; structural nuclear protein

Molecular Weight

Mass (kDA):
238.26 kDA

Genomic Context

Human
Location:	11q13.4
Sequence:	11; NC_000011.10 (72002864..72080693, complement)

Subcellular Localizations

Nucleus. Nucleus, nucleoplasm. Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cell cortex. Cell membrane; Lipid-anchor; Cytoplasmic side. Lateral cell membrane. Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826). The localization to the spindle poles is regulated by AAAS (PubMed:26246606). In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541

Diseases

• Malignant Neoplasms
• Neoplasms
• Leukemia
• Mammary Neoplasms
• Autoimmune Reaction
• Malignant Neoplasm Of Breast
• Autoimmune Diseases
• Aneuploidy
• Acute Promyelocytic Leukemia
• Malignant Paraganglionic Neoplasm
• Chromosomal Translocation
• Sjogren's Syndrome
• Leukemia, Myelocytic, Acute

• Myeloid Leukemia
• Lupus Erythematosus, Systemic
• Connective Tissue Diseases
• Bladder Neoplasm
• Malignant Neoplasm Of Urinary Bladder
• Adenocarcinoma
• Prostatic Neoplasms

Interacting Proteins

• CCDC57
• GNAI1
• GPSM2
• Gpsm2
• RAE1

• TERF2IP

Pathways

• Mitosis
• Interphase
• Localization
• Metaphase
• Cell Cycle
• Cell Division
• Spindle Assembly
• Anaphase
• Chromosome Segregation
• Cell Death
• Spindle Organization
• Telophase
• Transport

• Cytokinesis
• Asymmetric Cell Division
• Rna Interference
• Prophase
• Mitotic Spindle Organization
• Enucleation
• Chromatin Organization

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation

• Acetylation
• Methylation
• Ribosylation

Research Areas

• Breast Cancer
• Cell Biology
• Cell Cycle and Replication
• Cellular Markers
• Mitotic Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NUMA1

Best Uses Of The NUMA1 Marker

We will be discussing the Data analysis pipeline Josh Weinstein (David Scott) has created. This will allow you to find out whether your NUMA1 marker expression levels are higher or lower than the background population. This article includes results, discussion, conclusions, and recommendations. It is designed to help you make an informed choice about using your NUMA1 mark in your research. Boster Bio has more information on the pipeline.

David Scott, Josh Weinstein and Josh Weinstein created a data analysis process

The DKFZ data analysis pipeline was developed by David Scott and Josh Weinstein for their work at DKFZ (David Koch Foundation for Zoological Research). The team also credited other researchers such as Tobias Rausch, Kortine Kleinheinz, Jan O. Korbel, Ivo Buchhalter, Barbara Hutter, and Nagarajan Paramasivam. In addition, EMBL pipeline contributors include Matthias Schlesner, Joachim Weischenfeldt, and Yu Fan.

Results

This study was designed to determine whether the NUMA1 protein was essential for centrosome clustering. The nuclei in cells lacking AIS have a higher number of centrosomes. The USP28 depletion did not affect NuMA1 level. These findings suggest that NuMA1 may play a role in controlling the number and response of cells to DNA damage and the cellular response.

The AIS initiates action possibilities and regulates trafficking of vesicles among compartments. It is still not clear what mechanisms control AIS assembly. The assembly of AIS proteins is dependent on NuMA1, which binds with scaffolding protein 4.1B, and dynein regulator lissencephaly 1. NuMA1 increases their density. Its inhibition causes the depletion AIS protein, but not cell death.

NuMA1 also was found in both the pellet and soluble fractions. Cdc25C and Wee1 were found in smaller amounts in the pellet fraction of Wee1 siRNA-treated cells than in control cells. Metaphase cells had reduced levels of Cdc25C and cyclinB1 in their nuclei. These results were consistent to those of HeLa cells, which were also tested under similar experimental conditions.

Edit-CDK13-mutated cells had a nucleolus that was more abundant. This result shows that edit CDK13 decreases its association with nucleolus speckles. It also enriches TPC1 cells’ nucleolus. It also decreased association between nuclear rRNA. Further studies are needed before we can determine if NUMA1 mutant cells are more at risk for thyroid cancer.

The role of the nucleus in vascular remodelling is crucial. E11.5 embryonic mortality due to cardiovascular problems is caused when Notch2 is lost. Researchers can identify vascular-lesion-forming cells using unbiased protein profiling by using a mutant version (mutated) of this gene in humans. This study also shows queescence in smooth muscle cells of the human body is promoted by an increased Notch2.

In this study, P fraction of mitotic HeLacells was probed for the following antigens. P-fraction was enriched 10 times with S fraction. After incubation, cells were fixed in TBS-T with 5%-10% skimmilk. The cells were washed with PBS four times. Slides were coated with Mowiol 40-8 (Sigma-Aldol) to visualize the nuclei.

Discussions

Research in oncology revealed that the NUMA1 gene has been linked to many diseases. Among these diseases are acute promyelocytic leukemia, Kaposi's sarcoma, muscular dystrophy, and colorectal cancer. NUMA1 can also be associated with a host of gastrointestinal disorders including prostatitis, thyroiditis, esophagitis, and thyroiditis.

This polymorphic, non-synonymous nucleus in NuMA is located at coil three of its large central domain. NuMA performs many functions, including oligomerization or protein-protein interactions during Mitosis. This residue was previously found to have high homology with members of the SMC family of chromosome segregation ATPases. The risk of breast carcinogenesis is nearly doubled for those with the glycine mutation.

The study included 46 patient files containing NuMA1 markers. 24 patients had autoimmune disease. This included rheumatoid, lupus, and antiphospholipid syndrome. Devic Syndrome, which is linked with AD, was diagnosed in one patient. In addition, 15 patients had cardiovascular disease, autoimmune diseases, or other conditions. One patient had anti SSA or anti RNP antibodies. This may indicate a relationship.

The NuMA gene encodes an approximately 236 kDa protein with a large central domain of coiled coils. NuMA, a cell cycle-related proteins, is found in interphase's nuclear matrix. It also helps in spindle apparatus construction, chromosome position, and centrosome organization during mitosis. A NuMA antibody is useful in many aspects of cell biology.

Conclusions

The NUMA1 marker is the best candidate to visualize the protein distribution patterns in human tumor nucleoli. It is a widely used method for visualizing the distribution and localization of specific cellular components. It uses the interaction between antibodies and specific antigens in order to produce an intense signal which can be interpreted accordingly. This article discusses important findings from the Boster Bio study on the NUMA1 marker.