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Product Info Summary
| SKU: | A07249 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Acyl-protein thioesterase 1 LYPLA1 Antibody
SKU/Catalog Number
A07249
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Acyl-protein thioesterase 1 LYPLA1 Antibody (Catalog # A07249). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acyl-protein thioesterase 1 LYPLA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07249)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LYPLA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07249 is reactive to LYPLA1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
24670 MW
Background of LYPLA1
Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity.
Hu G.; Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Devedjiev Y., Structure 8:1137-1146(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07249 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, using LYPLA1 antibody A07249.
Protein Target Info & Infographic
Gene/Protein Information For LYPLA1 (Source: Uniprot.org, NCBI)
Gene Name
LYPLA1
Full Name
Acyl-protein thioesterase 1
Weight
24670 MW
Superfamily
AB hydrolase superfamily
Alternative Names
Acyl-protein thioesterase 1;APT-1;hAPT1;3.1.2.-;Lysophospholipase 1;Lysophospholipase I;LPL-I;LysoPLA I;LYPLA1;APT1, LPL1; LYPLA1 APT-1, APT1, LPL-I, LPL1, hAPT1 lysophospholipase 1 acyl-protein thioesterase 1|lysoPLA I|lysophospholipase I|lysophospholipid-specific lysophospholipase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LYPLA1, check out the LYPLA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LYPLA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acyl-protein thioesterase 1 LYPLA1 Antibody (A07249)
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No publications found for A07249
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Acyl-protein thioesterase 1 LYPLA1 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-LYPLA1 antibody A07249. Let me know if you need anything else.
G. Huang
Verified customer
Asked: 2019-12-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Is this A07249 anti-LYPLA1 antibody reactive to the isotypes of LYPLA1?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The immunogen of A07249 anti-LYPLA1 antibody is Synthesized peptide derived from internal of human LYPLA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-13
Question
Does A07249 anti-LYPLA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
As indicated on the product datasheet, A07249 anti-LYPLA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-06
Question
I see that the anti-LYPLA1 antibody A07249 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-18
Question
We are currently using anti-LYPLA1 antibody A07249 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
The anti-LYPLA1 antibody (A07249) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-28
Question
you antibody to test anti-LYPLA1 antibody A07249 on human brain for research purposes, then I may be interested in using anti-LYPLA1 antibody A07249 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Moore
Verified customer
Asked: 2016-07-01
Answer
The products we sell, including anti-LYPLA1 antibody A07249, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-07-01
Question
I was wanting to use your anti-LYPLA1 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
K. Walker
Verified customer
Asked: 2015-12-15
Answer
As indicated on the product datasheet, A07249 anti-LYPLA1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-15
