Anti-NT5C1A Antibody Picoband™

Product Name

Anti-NT5C1A Antibody Picoband™

View all NT5C1A Antibodies

SKU/Catalog Number

A12036-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NT5C1A Antibody Picoband™ catalog # A12036-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NT5C1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12036-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NT5C1A recombinant protein (Position: E2-Q368).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12036-2 is reactive to NT5C1A in Human

Applications

A12036-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

41 kDa

Calculated molecular weight

Background of NT5C1A

5'-nucleotidase, cytosolic IA is a protein that in humans is encoded by the NT5C1A gene. Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NT5C1A using anti-NT5C1A antibody (A12036-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5C1A antigen affinity purified polyclonal antibody (Catalog # A12036-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5C1A at approximately 41 kDa. The expected band size for NT5C1A is at 41 kDa.

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Figure 2. IHC analysis of NT5C1A using anti-NT5C1A antibody (A12036-2).
NT5C1A was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NT5C1A Antibody (A12036-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For NT5C1A (Source: Uniprot.org, NCBI)

Alternative Names

5'-nucleotidase, cytosolic IA; AMP-specific 5'-NT; CN1; CN1A; CN-I; CN-IA; cytosolic 5' nucleotidase, type 1A; cytosolic 5'-nucleotidase 1A; Cytosolic 5'-nucleotidase IA; EC 3.1.3.5; MGC119199; MGC119201 NT5C1A CN-I, CN-IA, CN1, CN1A, CNI 5-nucleotidase, cytosolic IA cytosolic 5-nucleotidase 1A|AMP-specific 5-NT|cytosolic 5 nucleotidase, type 1A|cytosolic 5-nucleotidase IA

For more info on NT5C1A, check out the NT5C1A Infographic

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In this infographic, you will see the following information for NT5C1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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