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Product Info Summary
| SKU: | M00078 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00078
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody catalog # M00078. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00078)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AGH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nrf2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00078 is reactive to NFE2L2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
67827 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00078 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
Positive Control
WB: 293T cell
IHC: human kidney tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Nrf2 expression in 293T cell treated with arsenite.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Nrf2 Antibody.
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Immunofluorescent analysis of Hela cells, using Nrf2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For NFE2L2 (Source: Uniprot.org, NCBI)
Gene Name
NFE2L2
Full Name
Nuclear factor erythroid 2-related factor 2
Weight
67827 MW
Superfamily
bZIP family
Alternative Names
Nuclear factor erythroid 2-related factor 2;NF-E2-related factor 2;NFE2-related factor 2;HEBP1;Nuclear factor, erythroid derived 2, like 2;NFE2L2;NRF2; NFE2L2 HEBP1, IMDDHH, NRF2, Nrf-2 nuclear factor, erythroid 2 like 2 nuclear factor erythroid 2-related factor 2|nuclear factor erythroid-derived 2-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFE2L2, check out the NFE2L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFE2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody (M00078)
Hello CJ!
M00078 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xin X,Yao D,Zhang K,Han S,Liu D,Wang H,Liu X,Li G,Huang J,Wang J.Protective effects of Rosavin on bleomycin-induced pulmonary fibrosis via suppressing fibrotic and inflammatory signaling pathways in mice.Biomed Pharmacother.2019 Jul;115:108870.doi:10.1016/j.biopha.2019.108870.Epub 2019 Apr 23.PMID:31026730.
Species: Mouse
M00078 usage in article: APP: WB, SAMPLE:LUNG TISSUE, DILUTION:1:400
Chen M,Wang Z,Zhou W,Lu C,Ji T,Yang W,Jin Z,Tian Y,Lei W,Wu S,Fu Q,Wu Z,Wu X,Han M,Fang M,Yang Y.SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells.Eur J Pharmacol.2021 Jun 8:174236.doi:10.1016/j.ejphar.2021.174236.Epub ahead of print.PMID:34116043.
Species: Mouse
M00078 usage in article: APP:WB, SAMPLE:N2A CELL, DILUTION:1:500
Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
M00078 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:400
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
M00078 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200
Li X,Zuo C,Sun D,Zhao T,Zhang Z.Arsenite Increases Linc-ROR in Human Bronchial Epithelial Cells that Can Be Inhibited by Antioxidant Factors.Biol Trace Elem Res.2020 Nov;198(1):131-141.doi:10.1007/s12011-020-02065-3.Epub 2020 Feb 6.PMID:32030632.
Species: Human
M00078 usage in article: APP:WB, SAMPLE:HBE CELL, DILUTION:1:500
Protective effects of berberine on renal injury in streptozotocin (STZ)-induced diabetic mice
miR-155 mediates arsenic trioxide resistance by activating Nrf2 and suppressing apoptosis in lung cancer cells
Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway
The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-?/Smad signaling and activation of the Nrf2/HO-1 pathway
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody
Question
Is this antibody produced by the baculovirus expression system?
Verified customer
Asked: 2021-12-10
Answer
No, the expression system of M00078 is plasmid transfection with 293F.
Boster Scientific Support
Answered: 2022-01-13
Question
Please let us know if this antibody contains the following substances in components: Infectious substances/ Toxin/pathogen (e.g. virus, bacterium, or microorganism）
Verified customer
Asked: 2021-12-10
Answer
NO, it does not include these substances.
Boster Scientific Support
Answered: 2021-12-13
Question
What is the clone name of M00078?
Verified customer
Asked: 2019-03-06
Answer
The clone name of Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody (M00078) is AGH-14.
Boster Scientific Support
Answered: 2019-03-06
