Product Info Summary
SKU: | P00078 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00078
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal Antibody catalog # P00078. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00078)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Nrf2 (S40)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00078 is reactive to NFE2L2 in Human, Mouse, Rat
Applications
P00078 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
100 kDa
Calculated molecular weight
67827 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
![p00078 nrf2 primary antibodies wb testing 1 p00078 nrf2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/p/0/p00078-nrf2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Nrf2 using anti-Nrf2 antibody (P00078).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nrf2 antigen affinity purified monoclonal antibody (Catalog # P00078) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nrf2 at approximately 100 kDa. The expected band size for Nrf2 is at 68 kDa.
![p00078 nrf2 primary antibodies ihc testing 2 p00078 nrf2 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/p/0/p00078-nrf2-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of Nrf2 using anti-Nrf2 antibody (P00078).
Nrf2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Nrf2 Antibody (P00078) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![p00078 nrf2 primary antibodies ihc testing 3 p00078 nrf2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/p/0/p00078-nrf2-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of Nrf2 using anti-Nrf2 antibody (P00078).
Nrf2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Nrf2 Antibody (P00078) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![p00078 nrf2 primary antibodies ihc testing 4 p00078 nrf2 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/p/0/p00078-nrf2-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of Nrf2 using anti-Nrf2 antibody (P00078).
Nrf2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Nrf2 Antibody (P00078) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![p00078 if p00078 if](https://www.bosterbio.com/media/catalog/product/p/0/p00078-if.jpg)
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Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.
![p00078 icc1 p00078 icc1](https://www.bosterbio.com/media/catalog/product/p/0/p00078-icc1.jpg)
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
![p00078 icc2 p00078 icc2](https://www.bosterbio.com/media/catalog/product/p/0/p00078-icc2.jpg)
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
![p00078 icc3 p00078 icc3](https://www.bosterbio.com/media/catalog/product/p/0/p00078-icc3.jpg)
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For NFE2L2 (Source: Uniprot.org, NCBI)
Gene Name
NFE2L2
Full Name
Nuclear factor erythroid 2-related factor 2
Weight
67827 MW
Superfamily
bZIP family
Alternative Names
HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2 ihc; Nrf2 immunofluorescence; Nrf2 immunohistochemistry; Nrf2 polyclonal; Nrf2 rat; Nrf2 western blot; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2 NFE2L2 HEBP1, IMDDHH, NRF2, Nrf-2 nuclear factor, erythroid 2 like 2 nuclear factor erythroid 2-related factor 2|nuclear factor erythroid-derived 2-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFE2L2, check out the NFE2L2 Infographic
![NFE2L2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFE2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal Antibody (P00078)
Hello CJ!
P00078 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Modulation of non‐steroidal anti‐inflammatory drug‐induced, ER stress‐mediated apoptosis in Caco‐2 cells by different polyphenolic antioxidants: a mechanistic study
Li X,Zuo C,Sun D,Zhao T,Zhang Z.Arsenite Increases Linc-ROR in Human Bronchial Epithelial Cells that Can Be Inhibited by Antioxidant Factors.Biol Trace Elem Res.2020 Nov;198(1):131-141.doi:10.1007/s12011-020-02065-3.Epub 2020 Feb 6.PMID:32030632.
Species: Human
P00078 usage in article: APP:WB, SAMPLE:HBE CELL, DILUTION:1:500
Protective effects of berberine on renal injury in streptozotocin (STZ)-induced diabetic mice
miR-155 mediates arsenic trioxide resistance by activating Nrf2 and suppressing apoptosis in lung cancer cells
Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal Antibody
Question
Will P00078 work on Western Blot of Bovine tissue lysates sample?
Verified customer
Asked: 2019-10-12
Answer
No, we don't have an anti-pNRF2 antibody that has been validated with Bovine samples.
Boster Scientific Support
Answered: 2019-10-16
Question
Has P00078 been tested on mouse tissues?
Verified customer
Asked: 2019-09-10
Answer
The Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal Antibody (P00078) is not recommended to be used on mouse samples.
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-Phospho-Nrf2 (S40) Rabbit Monoclonal antibody P00078 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
M. Miller
Verified customer
Asked: 2017-04-04
Answer
The anti-Phospho-Nrf2 (S40) Rabbit Monoclonal antibody (P00078) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-04