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Product Info Summary
| SKU: | PB9704 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FXYD1 Antibody Picoband®
View all Phospholemman Antibodies
SKU/Catalog Number
PB9704
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FXYD1 Antibody Picoband® catalog # PB9704. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FXYD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9704)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FXYD1 recombinant protein (Position: E21-R92). Human FXYD1 shares 86.1% and 87.5% amino acid (aa) sequence identity with mouse and rat FXYD1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9704 is reactive to FXYD1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
10 kDa
Calculated molecular weight
10441 MW
Background of Phospholemman
Phospholemman is a protein that in humans is encoded by the FXYD1 gene. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. Transcript variants with different 5' UTR sequences have been described in the literature.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9704 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Rat Cardiac Muscle Tissue, Mouse Cardiac Muscle Tissue, HEPG2 Whole Cell, HELA Whole Cell
IHC: mouse cardiac muscle tissue, rat cardiac muscle tissue, human prostatic cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug,
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug,
Lane 4: HEPG2 Whole Cell Lysate at 40ug,
Lane 5: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXYZ1 antigen affinity purified polyclonal antibody (Catalog # PB9704) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXYZ1 at approximately 10 kDa. The expected band size for FXYZ1 is at 10 kDa.
Click image to see more details
Figure 2. IHC analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704).
FXYZ1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FXYZ1 Antibody (PB9704) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704).
FXYZ1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FXYZ1 Antibody (PB9704) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704).
FXYZ1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FXYZ1 Antibody (PB9704) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For FXYD1 (Source: Uniprot.org, NCBI)
Gene Name
FXYD1
Full Name
Phospholemman
Weight
10441 MW
Superfamily
FXYD family
Alternative Names
Phospholemman;FXYD domain-containing ion transport regulator 1;FXYD1;PLM; FXYD1 PLM FXYD domain containing ion transport regulator 1 phospholemman|sodium/potassium-transporting ATPase subunit FXYD1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FXYD1, check out the FXYD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FXYD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FXYD1 Antibody Picoband® (PB9704)
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Customer Q&As
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5 Customer Q&As for Anti-FXYD1 Antibody Picoband®
Question
We are currently using anti-FXYD1 antibody PB9704 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-FXYD1 antibody (PB9704) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
Is this PB9704 anti-FXYD1 antibody reactive to the isotypes of FXYD1?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The immunogen of PB9704 anti-FXYD1 antibody is E.coli-derived human FXYD1 recombinant protein (Position: E21-R92). Human FXYD1 shares 86.1% and 87.5% amino acid (aa) sequence identity with mouse and rat FXYD1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-FXYD1 antibody PB9704. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-04
Question
Please see the WB image, lot number and protocol we used for brain using anti-FXYD1 antibody PB9704. Please let me know if you require anything else.
S. Zhang
Verified customer
Asked: 2018-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-20
Question
My question regarding product PB9704, anti-FXYD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9704 anti-FXYD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-13
