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Product Info Summary
| SKU: | A00462-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Toll-like receptor 5 TLR5 Antibody
SKU/Catalog Number
A00462-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Toll-like receptor 5 TLR5 Antibody (Catalog # A00462-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
TLR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Toll-like receptor 5 TLR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00462-1)
Host
Rabbit
Contents
TLR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5. The immunogen is located within the last 50 amino acids of TLR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1.
Reactive Species
A00462-1 is reactive to TLR5 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
97834 MW
Background of TLR5
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-α and other cytokines. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires'disease and demonstrates the importance of TLR5 in the innate immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00462-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TLR5 antibody can be used for detection of TLR5 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TLR5 expression in HT29 cell lysate with TLR5 antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of TLR5 in THP-1 cells with TLR5 antibody at 10 μg/mL.
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Immunofluorescence of TLR5 in THP1 cells with TLR5 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TLR5 (Source: Uniprot.org, NCBI)
Gene Name
TLR5
Full Name
Toll-like receptor 5
Weight
97834 MW
Superfamily
Toll-like receptor family
Alternative Names
TIL3, SLEB1, MELIOS, TIL3, Toll-like receptor 5 TLR5 MELIOS, SLE1, SLEB1, TIL3 toll like receptor 5 toll-like receptor 5|systemic lupus erythematosus susceptibility 1|toll/interleukin-1 receptor-like protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR5, check out the TLR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Toll-like receptor 5 TLR5 Antibody (A00462-1)
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