Anti-Psmb1 Antibody Picoband™

Product Name

Anti-Psmb1 Antibody Picoband™

View all Proteasome beta 1 Antibodies

SKU/Catalog Number

A06891-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Psmb1 Antibody Picoband™ catalog # A06891-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Psmb1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06891-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Psmb1 recombinant protein (Position: S21-D240).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06891-1 is reactive to PSMB1 in Mouse, Rat

Applications

A06891-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

87148 MW

Background of Proteasome beta 1

Proteasome subunit beta type-1 also known as 20S proteasome subunit beta-6 (based on systematic nomenclature) is a protein that in humans is encoded by the PSMB1 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Psmb1 using anti-Psmb1 antibody (A06891-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Psmb1 antigen affinity purified polyclonal antibody (Catalog # A06891-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Psmb1 at approximately 26 kDa. The expected band size for Psmb1 is at 26 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-Psmb1 antibody (A06891-1).
Overlay histogram showing RAW264.7 cells stained with A06891-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Psmb1 Antibody (A06891-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For PSMB1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.25.1; FLJ25321; HC5; KIAA1838; Macropain subunit C5; Multicatalytic endopeptidase complex subunit C5; PMSB1; proteasome (prosome, macropain) subunit, beta type, 1; proteasome beta 1 subunit; Proteasome component C5; Proteasome gamma chain; proteasome subunit beta type-1; proteasome subunit HC5; PSC5 PSMB1 HC5, PMSB1, PSC5 proteasome 20S subunit beta 1 proteasome subunit beta type-1|macropain subunit C5|multicatalytic endopeptidase complex subunit C5|proteasome (prosome, macropain) subunit, beta type, 1|proteasome beta 1 subunit|proteasome component C5|proteasome gamma chain|proteasome subunit HC5|proteasome subunit beta 1|proteasome subunit beta6|testicular secretory protein Li 45

For more info on PSMB1, check out the PSMB1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PSMB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the PSMB1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis