Anti-NR2F6 Purified Monoclonal Antibody

Product Name

Anti-NR2F6 Purified Monoclonal Antibody

View all EAR2/NR2F6 Antibodies

SKU/Catalog Number

M06990

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NR2F6 Purified Monoclonal Antibody (Catalog# M06990). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-NR2F6 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06990)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EM-51

Isotype

Mouse IgG2a kappa

Immunogen

Recombinant human NR2F6 protein expressed in E. coli. The mouse monoclonal antibody EM-51 recognizes NR2F6, a transcriptional repressor (intracellular antigen) expressed mainly in the heart, placenta, liver, skeletal muscle, kidney and pancreas, but also e.g. in T cell subpopulations.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M06990 is reactive to NR2F6 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

42979 MW

Background of EAR2/NR2F6

NR2F6 (nuclear receptor subfamily 2 group F member 6), also known as EAR2 or ERBAL2, is a transcription factor involved in modulation of hormonal responses. NR2F6 represses e.g. transcription of the oxytocin-neurophysin gene, renin gene, lutropin-choriogonadotropic hormone receptor gene, and the thyroid hormone receptor gene. In the immune system, NR2F6 affects IL-17 expression in the Th-17 cells, thus also the balance between immunological tolerance and autoimmunity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06990 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: Positive control: HEK293T/17/NR2F6/Ha-tag transfectants, negative control: HEK293T/17 cells.
Flow cytometry: 3-9 µg/ml. Intracellular staining.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For NR2F6 (Source: Uniprot.org, NCBI)

Alternative Names

EAR2, EAR-2, ERBAL2 NR2F6 EAR-2, EAR2, ERBAL2 nuclear receptor subfamily 2 group F member 6 nuclear receptor subfamily 2 group F member 6|ERBA-related gene-2|V-erbA-related protein 2|nuclear receptor V-erbA-related|v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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