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Product Info Summary
| SKU: | PB10081 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NR2F6 Antibody Picoband®
View all EAR2/NR2F6 Antibodies
SKU/Catalog Number
PB10081
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NR2F6 Antibody Picoband® catalog # PB10081. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR2F6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10081)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human NR2F6 recombinant protein (Position: M270-Q404). Human NR2F6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat NR2F6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10081 is reactive to NR2F6 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
42979 MW
Background of EAR2/NR2F6
Nuclear receptor subfamily 2 group F member 6 is a protein that in humans is encoded by the NR2F6 gene. It is a member of the nuclear receptor family of intracellular transcription factors. It has been shown to function as a coregulator of other nuclear receptors. NR2F6 impairs the formation of mature red blood cells in animals that over-express NR2F6 in their bone marrow. Mice that over expression of NR2F6 in their bone marrow cells have a block at the pro-erythroblast stage of blood cell development both in the bone marrow and in the spleen of animals that have excessive expression of NR2F6. So, when inhibition of differentiation of stem cell is desired, inhibition of differentiation is achieved through induction of increased NR2F6 activity. In situations where differentiation of stem cells into a cell of increased maturity is desired, inhibition of NR2F6 activity must be performed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10081 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat liver tissue, HELA whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NR2F6 using anti-NR2F6 antibody (PB10081).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR2F6 antigen affinity purified polyclonal antibody (Catalog # PB10081) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR2F6 at approximately 43 kDa. The expected band size for NR2F6 is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NR2F6 (Source: Uniprot.org, NCBI)
Gene Name
NR2F6
Full Name
Nuclear receptor subfamily 2 group F member 6
Weight
42979 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Nuclear receptor subfamily 2 group F member 6;V-erbA-related protein 2;EAR-2;NR2F6;EAR2, ERBAL2; NR2F6 EAR-2, EAR2, ERBAL2 nuclear receptor subfamily 2 group F member 6 nuclear receptor subfamily 2 group F member 6|ERBA-related gene-2|V-erbA-related protein 2|nuclear receptor V-erbA-related|v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR2F6, check out the NR2F6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR2F6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR2F6 Antibody Picoband® (PB10081)
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Customer Q&As
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5 Customer Q&As for Anti-NR2F6 Antibody Picoband®
Question
We are currently using anti-NR2F6 antibody PB10081 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on dog tissues as well?
D. Walker
Verified customer
Asked: 2019-05-06
Answer
The anti-NR2F6 antibody (PB10081) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-06
Question
Is this PB10081 anti-NR2F6 antibody reactive to the isotypes of NR2F6?
K. Mangal
Verified customer
Asked: 2017-10-17
Answer
The immunogen of PB10081 anti-NR2F6 antibody is E. coli-derived human NR2F6 recombinant protein (Position: M270-Q404). Human NR2F6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat NR2F6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-17
Question
Is there a BSA free version of anti-NR2F6 antibody PB10081 available?
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NR2F6 antibody PB10081 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-29
Question
you antibody to test anti-NR2F6 antibody PB10081 on human fetal lung for research purposes, then I may be interested in using anti-NR2F6 antibody PB10081 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Zhao
Verified customer
Asked: 2016-05-20
Answer
The products we sell, including anti-NR2F6 antibody PB10081, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-20
Question
I see that the anti-NR2F6 antibody PB10081 works with WB, what is the protocol used to produce the result images on the product page?
M. Krishna
Verified customer
Asked: 2015-01-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-01-14
