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Product Info Summary
| SKU: | EK0369 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA, citrate). |
Product info
Product Name
Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®
SKU/Catalog Number
EK0369
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® (96 Tests). Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0369)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)
Sensitivity
<5 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0369 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0369 is reactive to HGF in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA, citrate).
Application Guarantee
EK0369 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HGF
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0369-CAP | Anti-Human HGF Pre-coated 96-well strip microplate | 1 |
| EK0369-ST | Human HGF Standard | 2 vials, 10 ng/tube |
| EK0369-DA | Human HGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.014 | 0.081 | 0.116 | 0.217 | 0.357 | 0.631 | 1.261 | 2.033 |
Data Example Images
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Human HGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HGF, For serum, dilution ratio is 1:1 and concentration is 799-3642 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 375-1946 pg/ml..
Some articles we found to cite concentrations of HGF in samples: 25697336 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 169 | 1246 | 4183 | 168 | 1364 | 4239 |
| Standard deviation | 9.8 | 87.22 | 267.71 | 9.91 | 118.66 | 313.68 |
| CV (%) | 5.8% | 7% | 6.4% | 5.9% | 8.7% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 169 | 159 | 165 | 152 | 161 | 6.41 | 3.9% |
| Sample 2 | 1246 | 1103 | 1132 | 1240 | 1180 | 63.61 | 5.3% |
| Sample 3 | 4183 | 3905 | 4308 | 3657 | 4013 | 252.15 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For HGF (Source: Uniprot.Org, NCBI)
Gene Name
HGF
Full Name
Hepatocyte growth factor
Weight
83134 MW
Superfamily
peptidase S1 family
Alternative Names
Hepatocyte growth factor;Hepatopoietin-A;Scatter factor;SF;Hepatocyte growth factor alpha chain;Hepatocyte growth factor beta chain;HGF;HPTA; HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on HGF, check out the HGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® (EK0369)
Hello CJ!
EK0369 has been cited in 22 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Role of Hepatocyte Growth Factor in the Immunomodulation Potential of Amniotic Fluid Stem Cells
Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations
Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin
Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor
Cucurbit[8]uril Supramolecular Assembly for Positively Charged Ultrathin Films as Nanocontainers
Substrate-mediated delivery of gene complex nanoparticles via polydopamine coating for enhancing competitiveness of endothelial cells
Utility of Hepatocyte Growth Factor as a Biomarker for Early Diagnosis of Pulmonary Artery Hypertension
Serial changes of cytokines and growth factors in peripheral circulation after right lobe donor hepatectomy
Exosomes derived from adipose tissue, bone marrow, and umbilical cord blood for cardioprotection after myocardial infarction
The (PrS/HGF‐pDNA) multilayer films for gene‐eluting stent coating: Gene‐protecting, anticoagulation, antibacterial properties, and in vivo antirestenosis evaluation
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Customer Reviews
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Customer Q&As
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21 Customer Q&As for Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®
Question
What is the lower limit of quantification (LLOQ) and measuring absorbance (wavelength) of EK0369?
Verified customer
Asked: 2022-05-06
Answer
For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the lower limit of quantification (LLOQ) is 125pg/ml and measuring absorbance (wavelength) is 450nm.
Boster Scientific Support
Answered: 2022-05-07
Question
What tissue types have you tested with EK0369?
Verified customer
Asked: 2022-05-06
Answer
Our lab hasn't tested any tissue with the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369). Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first; Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2022-05-07
Question
What plasma/serum concentration did you see in your samples when preparing EK0369?
Verified customer
Asked: 2022-05-06
Answer
The plasma/serum concentration observed is 300-3300pg/ml.
Boster Scientific Support
Answered: 2022-05-07
Question
Is there a recommended dilution factor for plasma/serum samples using EK0369?
Verified customer
Asked: 2022-05-06
Answer
The recommended dilution factor for the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369) is 1:1.
Boster Scientific Support
Answered: 2022-05-07
Question
What is the Host Species for the capture and detection antibodies of EK0369?
Verified customer
Asked: 2022-05-06
Answer
For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the Host Species for the capture antibody is mouse while the Host Species for the detection antibody is goat.
Boster Scientific Support
Answered: 2022-05-07
Question
What is the Isotype for the capture and detection antibodies of EK0369?
Verified customer
Asked: 2022-05-06
Answer
For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the capture antibody Isotype is IgG1 while the detection antibody Isotype is IgG.
Boster Scientific Support
Answered: 2022-05-07
Question
What is the clonality for the capture and detection antibodies of EK0369?
Verified customer
Asked: 2022-05-06
Answer
For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the capture antibody clonality is monoclonal while the detection antibody clonality is polyclonal.
Boster Scientific Support
Answered: 2022-05-07
Question
What is the peptide sequence or general region of the protein used for EK0369?
Verified customer
Asked: 2022-05-06
Answer
The peptide sequence or general region of the protein used for EK0369 is Q32-R494 (alpha) & V495-S728 (beta).
Boster Scientific Support
Answered: 2022-05-07
Question
What expression system was used in EK0369 if the immunogen is a recombinant protein?
Verified customer
Asked: 2022-05-06
Answer
NS0 was used for the standard of the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369).
Boster Scientific Support
Answered: 2022-05-07
Question
What is the sensitivity of EK0369?
Verified customer
Asked: 2022-05-06
Answer
The Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369) sensitivity is <5 pg/ml.
Boster Scientific Support
Answered: 2022-05-07
Question
Has the cross-reactivity of EK0369 been tested?
Verified customer
Asked: 2022-05-06
Answer
Our lab tested the recombinant proteins listed below using the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369) and no obvious cross-reactivity was observed. Recombinant human: EGF, G-CSF, GM-CSF, HGF R, M-CSF, Met, PDGF-AA, PDGF-AB, PDGF-BB, VEGF
Boster Scientific Support
Answered: 2022-05-07
Question
Q: if the enzyme conjugated HGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HGF antigen?
D. Sunder
Verified customer
Asked: 2020-04-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: how do I analyze ELISA data? I have performed HGF level in plasma.
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-14
Question
Does EK0369 cross react with mouse?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
EK0369 does not cross react with mouse.
Boster Scientific Support
Answered: 2019-09-26
Question
Does EK0369 detect the active form of HGF?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
EK0369 is able to detect the active form of HGF.
Boster Scientific Support
Answered: 2019-06-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Kaur
Verified customer
Asked: 2018-09-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-09-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Y. Zhao
Verified customer
Asked: 2018-09-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-09-03
Question
Q: can I use heparin plasma as samples in Human HGF Picokine® ELISA Kit (Catalog # EK0369)?
C. Jha
Verified customer
Asked: 2018-06-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of HGF causing degradation of its protein structure. HGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the HGF ELISA, treating samples with a number of anticoagulants and decided that EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-06-21
Question
I hope that you week is going well! I have a customer interested in this product but has a few questions. Could you assist? Could you let me know if: 1) the antibodies are monoclonal or polyclonal? 2) Whether cross species reactivity to mouse has been checked? 3) Does this kit work on tissue lysate?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. EK0369 doesn't cross react with mouse.
Boster Scientific Support
Answered: 2017-07-21
Question
Q: can you recommend the dilution ratio of serum samples for detection of HGF in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
A. Campbell
Verified customer
Asked: 2014-11-29
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the HGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-11-29
Question
Is this kit able to detect the active form of HGF?
Verified Customer
Verified customer
Asked: 2013-05-10
Answer
EK0369 is able to detect the active form of HGF.
Boster Scientific Support
Answered: 2013-05-10
