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Product Info Summary
| SKU: | A08389-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NPFFR2 Antibody Picoband®
View all GPR74/NPFFR2 Antibodies
SKU/Catalog Number
A08389-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NPFFR2 Antibody Picoband® catalog # A08389-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NPFFR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08389-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NPFFR2 recombinant protein (Position: L14-E521).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08389-2 is reactive to NPFFR2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
82457 MW
Background of GPR74/NPFFR2
Neuropeptide FF receptor 2, also known as NPFF2 is a human protein encoded by the NPFFR2 gene. This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08389-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NPFFR2 using anti-NPFFR2 antibody (A08389-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPFFR2 antigen affinity purified polyclonal antibody (Catalog # A08389-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPFFR2 at approximately 60 kDa. The expected band size for NPFFR2 is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NPFFR2 (Source: Uniprot.org, NCBI)
Gene Name
NPFFR2
Full Name
Neuropeptide FF receptor 2
Weight
82457 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 2;ADAM 2;Cancer/testis antigen 15;CT15;Fertilin subunit beta;PH-30;PH30;PH30-beta;ADAM2;FTNB; NPFFR2 GPR74, HLWAR77, NPFF2, NPGPR neuropeptide FF receptor 2 neuropeptide FF receptor 2|G-protein coupled receptor 74|G-protein coupled receptor HLWAR77|neuropeptide FF 2|neuropeptide G-protein coupled receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPFFR2, check out the NPFFR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPFFR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NPFFR2 Antibody Picoband® (A08389-2)
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