Anti-U2AF65/U2AF2 Antibody Picoband®

U2AF2 antibody

Boster Bio Anti-U2AF65/U2AF2 Antibody Picoband® catalog # A03639-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A03639-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-U2AF65/U2AF2 Antibody Picoband®

View all U2AF2 Antibodies

SKU/Catalog Number

A03639-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-U2AF65/U2AF2 Antibody Picoband® catalog # A03639-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-U2AF65/U2AF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03639-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human U2AF65/U2AF2 recombinant protein (Position: M238-H470).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03639-2 is reactive to U2AF2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

65 kDa

Calculated molecular weight

28461 MW

Background of U2AF2

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03639-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human HEK293 whole cell, human THP-1 whole cell, human U2OS whole cell, rat brain tissue, rat spleen tissue, mouse brain tissue
IHC: human mammary cancer tissue, mouse spleen tissue, rat spleen tissue
ICC/IF: Hela cell
FCM: PC-3 cell, ANA-1 cell, NRK cell

Validation Images & Assay Conditions

Gene/Protein Information For U2AF2 (Source: Uniprot.org, NCBI)

Gene Name

U2AF2

Full Name

Splicing factor U2AF 65 kDa subunit

Weight

28461 MW

Superfamily

splicing factor SR family

Alternative Names

Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit; hU2AF (65); Hu2af65; U2 snRNP auxiliary factor large subunit; U2AF2; U2AF65 U2AF2 U2AF65 U2 small nuclear RNA auxiliary factor 2 splicing factor U2AF 65 kDa subunit|U2 (RNU2) small nuclear RNA auxiliary factor 2|U2 auxiliary factor 65 kDa subunit|U2 small nuclear ribonucleoprotein auxiliary factor (65kD)|U2 snRNP auxiliary factor large subunit|hU2AF65

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on U2AF2, check out the U2AF2 Infographic

U2AF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for U2AF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03639-2

100μg

$370
A03639-2-10ug

10μg sample (liquid)

$99
A03639-2-Biotin

100 μg Biotin conjugated

$570
A03639-2-Cy3

100 μg Cy3 conjugated

$570
A03639-2-Dylight488

100 μg Dylight488 conjugated

$570
A03639-2-Dylight550

100 μg Dylight550 conjugated

$570
A03639-2-Dylight594

100 μg Dylight594 conjugated

$570
A03639-2-FITC

100 μg FITC conjugated

$570
A03639-2-HRP

100 μg HRP conjugated

$570
A03639-2-APC

100 μg APC conjugated

$670
A03639-2-PE

100 μg PE conjugated

$670
A03639-2-iFluor647

100 μg iFluor647 conjugated

$670
A03639-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A03639-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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