Anti-NADPH oxidase organizer 1 NOXO1 Antibody

Product Name

Anti-NADPH oxidase organizer 1 NOXO1 Antibody

View all Noxo1 Antibodies

SKU/Catalog Number

A05047

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NADPH oxidase organizer 1 NOXO1 Antibody (Catalog # A05047). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-NADPH oxidase organizer 1 NOXO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05047)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05047 is reactive to NOXO1 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

38.827kDa

Background of Noxo1

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05047 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 5-10 µg/ml
WB: 1:1,000 - 1:10,000

Validation Images & Assay Conditions

Gene/Protein Information For NOXO1 (Source: Uniprot.org, NCBI)

Alternative Names

NOXO1, NOXO-1, NOXO 1, NADPH oxidase organizer 1, NADPH oxidase regulatory protein, Nox organizer 1, Nox organizing protein 1, Regulatory protein P41NOX, SH3 and PX domain-containing protein 5, P41NOX, SH3PXD5 Noxo1|2310034C04Rik, P41NOX, P41NOXA, P41NOXB, P41NOXC, Snx28, hslt|NADPH oxidase organizer 1|NADPH oxidase organizer 1|nox organizer 1|sorting nexin-28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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