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Product Info Summary
| SKU: | PA1930 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NOXA1 Antibody Picoband®
SKU/Catalog Number
PA1930
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NOXA1 Antibody catalog # PA1930. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOXA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1930)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOXA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1930 is reactive to NOXA1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
51 kDa
Background of NOXA1
NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1930 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U87 whole cell, human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NOXA1 using anti-NOXA1 antibody (PPA1930).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA1 antigen affinity purified polyclonal antibody (Catalog # PA1930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA1 at approximately 60 kDa. The expected band size for NOXA1 is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NOXA1 (Source: Uniprot.org, NCBI)
Gene Name
NOXA1
Full Name
NADPH oxidase activator 1
Weight
51 kDa
Superfamily
NCF2/NOXA1 family
Alternative Names
NADPH oxidase activator 1;NOX activator 1;Antigen NY-CO-31;NCF2-like protein;P67phox-like factor;p51-nox;NOXA1;P51NOX; NOXA1 NY-CO-31, SDCCAG31, p51NOX NADPH oxidase activator 1 NADPH oxidase activator 1|NCF2-like protein|Nox activator 1| NY-CO-31|inhibitory NADPH oxidase activator 1|p51-nox|p67phox-like factor|serologically defined colon cancer 31
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOXA1, check out the NOXA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOXA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOXA1 Antibody Picoband® (PA1930)
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Customer Q&As
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4 Customer Q&As for Anti-NOXA1 Antibody Picoband®
Question
We are currently using anti-NOXA1 antibody PA1930 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-NOXA1 antibody (PA1930) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
Would anti-NOXA1 antibody PA1930 work for WB with colon cancer?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
According to the expression profile of colon cancer, NOXA1 is highly expressed in colon cancer. So, it is likely that anti-NOXA1 antibody PA1930 will work for WB with colon cancer.
Boster Scientific Support
Answered: 2019-07-15
Question
I see that the anti-NOXA1 antibody PA1930 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-12
Question
We need to test anti-NOXA1 antibody PA1930 on human colon cancer for research purposes, then I may be interested in using anti-NOXA1 antibody PA1930 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Edwards
Verified customer
Asked: 2019-01-30
Answer
The products we sell, including anti-NOXA1 antibody PA1930, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-30
