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Product Info Summary
| SKU: | PA1946 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NFkB/NFKB2 p100/p52 Antibody Picoband®
SKU/Catalog Number
PA1946
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFkB/NFKB2 p100/p52 Antibody catalog # PA1946. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1946)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse NFkB/NFKB2 p100/p52, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1946 is reactive to Nfkb2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
96832 MW
Background of NFKB2
NFKB2 (Nuclear Factor Kappa-B, Subunit 2), also known as NFKB or p52/p100 SUBUNIT, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al. (1992) mapped the gene for what they called the p49/p100 subunit of NFKB (NFKB2) to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization (FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al. (2002) showed that bone marrow (BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1 (T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of Nfkb2 to p52, which again required Baffr and Nik, but not Nemo (IKKG). Immunoblot analysis showed that BM cells contained primarily p100.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1946 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Mouse Liver Tissue, HEPA Cell
IHC: Rat Intestine Tissue
Validation Images & Assay Conditions
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Anti-NFkB p100/p52 antibody, PA1946, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-NFkB p100/p52 antibody, PA1946, Western blotting
Lane 1: Mouse Liver Tissue Lysate
Lane 2: HEPA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Nfkb2 (Source: Uniprot.org, NCBI)
Gene Name
Nfkb2
Full Name
Nuclear factor NF-kappa-B p100 subunit
Weight
96832 MW
Alternative Names
Nuclear factor NF-kappa-B p100 subunit;DNA-binding factor KBF2;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2;Nuclear factor NF-kappa-B p52 subunit;Nfkb2; NFKB2 CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52 nuclear factor kappa B subunit 2 nuclear factor NF-kappa-B p100 subunit|DNA-binding factor KBF2|NFKB, p52/p100 subunit|lymphocyte translocation chromosome 10 protein|nuclear factor Kappa-B, subunit 2|nuclear factor NF-kappa-B p52 subunit|nuclear factor of Kappa light chain gene enhancer in B cells 2|nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)|oncogene Lyt-10|transcription factor NFKB2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nfkb2, check out the Nfkb2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nfkb2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® (PA1946)
Hello CJ!
PA1946 has been cited in 23 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dynamic activation of the key pathways: linking colitis to colorectal cancer in a mouse model
Naringin attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress and NF-%u03BAB activation in vivo and in vitro
Therapeutic effect of artemisinin on lupus nephritis mice and its mechanisms
Ursolic acid protects against ulcerative colitis via anti-inflammatory and antioxidant effects in mice
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats
Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects
Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy.
Ozone preconditioning and exposure to ketamine attenuates hepatic inflammation in septic rats
Neuroprotective effect of combining tanshinone IIA with low-dose methylprednisolone following acute spinal cord injury in rats
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