Product Info Summary
SKU: | FEK0904 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Assay Time: | 120 Minutes |
Customers Who Bought This Also Bought
Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit
See all CEACAM5/CD66e products
SKU/Catalog Number
FEK0904
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0904)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: K35-A685
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0904 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0904 is reactive to CEACAM5 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK0904 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CEACAM5/CD66e
Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0904-CAP | 96-well plate precoated with anti-Human CEACAM5/CD66e antibody | 1 |
EK0904-ST | lyophilized recombinant Human CEACAM5/CD66e standard | 20 ng/tube |
FEK0904-DA | Biotinylated anti-Human CEACAM5/CD66e detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.011 | 0.056 | 0.111 | 0.240 | 0.381 | 0.713 | 1.296 | 1.967 |
Data Example Images
Click image to see more details
Human CEA PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 776 | 1668 | 10176 | 724 | 1662 | 10501 |
Standard deviation | 39.57 | 124 | 407.04 | 39.09 | 137.94 | 420.04 |
CV (%) | 4% | 7.5% | 4% | 5.4% | 8.3% | 4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CEACAM5/CD66e in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 776 | 816 | 775 | 762 | 782 | 20.25 | 2.5% |
Sample 2 | 1668 | 1752 | 1723 | 1646 | 1697 | 42.25 | 2.4% |
Sample 3 | 10176 | 9323 | 9418 | 9597 | 9628 | 331.05 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For CEACAM5 (Source: Uniprot.Org, NCBI)
Uniprot ID
P06731
Gene ID
1048
Gene Name
CEACAM5
Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Weight
76795 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA; CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic -related cell adhesion molecule 5|carcinoembryonic related cell adhesion molecule 5|meconium 100
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CEACAM5, check out the CEACAM5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CEACAM5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (FEK0904)
Hello CJ!
No publications found for FEK0904
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit
Question
Dear Technical Support: I would like to purchase FEK0904 antibody but I would like to ask if it is strip type or one plate type?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our product FEK0904 antibody comes with the 96-well strip plate (12x8) and each strip (12) has 8 wells, so you can use strip at a time.
Boster Scientific Support
Answered: 2019-08-12