Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit

CEACAM5/CD66e ELISA kit, 120-minute protocol

Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: FEK0904
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit

See all CEACAM5/CD66e products

SKU/Catalog Number

FEK0904

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0904)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: K35-A685

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of FEK0904 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0904 is reactive to CEACAM5 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0904 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CEACAM5/CD66e

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Kit Components

Catalog number Description Quantity
FEK0904-CAP 96-well plate precoated with anti-Human CEACAM5/CD66e antibody 1
EK0904-ST lyophilized recombinant Human CEACAM5/CD66e standard 20 ng/tube
FEK0904-DA Biotinylated anti-Human CEACAM5/CD66e detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0110.0560.1110.2400.3810.7131.2961.967

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)776166810176724166210501
Standard deviation39.57124407.0439.09137.94420.04
CV (%)4%7.5%4%5.4%8.3%4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CEACAM5/CD66e in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 177681677576278220.252.5%
Sample 21668175217231646169742.252.4%
Sample 3101769323941895979628331.053.4%
*number of samples for each test n=16.

Gene/Protein Information For CEACAM5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06731

Gene ID

1048

Gene Name

CEACAM5

Full Name

Carcinoembryonic antigen-related cell adhesion molecule 5

Weight

76795 MW

Superfamily

immunoglobulin superfamily

Alternative Names

Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA; CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic -related cell adhesion molecule 5|carcinoembryonic related cell adhesion molecule 5|meconium 100

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CEACAM5, check out the CEACAM5 Infographic

CEACAM5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CEACAM5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit

Question

Dear Technical Support: I would like to purchase FEK0904 antibody but I would like to ask if it is strip type or one plate type?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Our product FEK0904 antibody comes with the 96-well strip plate (12x8) and each strip (12) has 8 wells, so you can use strip at a time.

Boster Scientific Support

Answered: 2019-08-12

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