Human Carcinoembryonic Antigen (CEA) OneStep ELISA Kit

Product Name

Human Carcinoembryonic Antigen (CEA) OneStep ELISA Kit

See all CEACAM5/CD66e products

SKU/Catalog Number

EK7031

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Carcinoembryonic Antigen (CEA). 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Carcinoembryonic Antigen (CEA) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7031)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2ng/ml

Assay Range

5-120ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7031 is reactive to CEACAM5 in Human samples

Validated Sample Types

Serum and Plasma (heparin, citrate)

Application Guarantee

EK7031 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human Carcinoembryonic Antigen (CEA) Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti- Carcinoembryonic Antigen (CEA) monoclonal antibody, Polystyrene micro-well plate
Human Carcinoembryonic Antigen (CEA) Standards (S0~S5)	6	1ml	Carcinoembryonic Antigen (CEA) (0，5，10, 30, 60, 120) ng/ml, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300, from natural protein
HRP Conjugated anti-Human Carcinoembryonic Antigen (CEA) antibody	1	7ml	HRP Conjugated anti-Human Carcinoembryonic Antigen (CEA) antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1% Proclin-300, from mouse monoclonal antibody
Controls	2	1ml	Carcinoembryonic Antigen (CEA) L（5.25-9.75）H (17.5-32.5) ng/ml, 100% natural protein, 0.1% Proclin-300, from natural protein
20X Wash Buffer Concentrate	1	15ml	Phosphorous salts
Color Developing Reagent A	1	7ml	Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	TMB
Stop Solution	1	7ml	Dilute sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis