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Product Info Summary
| SKU: | M11954 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody
View all RBFOX3/NeuN Antibodies
SKU/Catalog Number
M11954
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody catalog # M11954. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11954)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AO-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NeuN
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M11954 is reactive to RBFOX3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
33873 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11954 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human fetal brain, rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, Rat testis tissue, Human Hodgkin's lymphoma tissue, Human astrocytoma tissue, Human stomach tissue, Mouse testis tissue
Validation Images & Assay Conditions
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Western blot analysis of NeuN expression in human fetal brain lysate.
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Western blot analysis of NeuN using anti-NeuN antibody (M11954).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NeuN antigen affinity purified monoclonal antibody (Catalog # M11954) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NeuN at approximately 46-55 kDa. The expected band size for NeuN is at 34 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using NeuN Antibody.
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Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse testis, using the Antibody at 1:1000 dilution.
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Immunofluorescent analysis of SH-SY5Y cells, using NeuN Antibody .
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For RBFOX3 (Source: Uniprot.org, NCBI)
Gene Name
RBFOX3
Full Name
RNA binding protein fox-1 homolog 3
Weight
33873 MW
Alternative Names
RNA binding protein fox-1 homolog 3;Fox-1 homolog C;RBFOX3; Rbfox3|Fox-3, Hrn, Hrnbp3, Ne, NeuN, Neuna60|RNA binding protein, fox-1 homolog (C. elegans) 3|RNA binding protein fox-1 homolog 3|fox-1 homolog C|hexaribonucleotide binding protein 3|neuN |neuronal nuclear A60|neuronal nuclei
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBFOX3, check out the RBFOX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBFOX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody (M11954)
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Customer Q&As
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5 Customer Q&As for Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody
Question
I see that the anti-NeuN Rabbit Monoclonal antibody M11954 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-29
Question
Please see the WB image, lot number and protocol we used for cerebellum using anti-NeuN Rabbit Monoclonal antibody M11954. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-03
Question
Is a blocking peptide available for product anti-NeuN Rabbit Monoclonal antibody (M11954)?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
We do provide the blocking peptide for product anti-NeuN Rabbit Monoclonal antibody (M11954). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-30
Question
My lab would like to test anti-NeuN Rabbit Monoclonal antibody M11954 on mouse cerebellum for research purposes, then I may be interested in using anti-NeuN Rabbit Monoclonal antibody M11954 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Parker
Verified customer
Asked: 2016-08-16
Answer
The products we sell, including anti-NeuN Rabbit Monoclonal antibody M11954, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-16
Question
Will anti-NeuN Rabbit Monoclonal antibody M11954 work for IF with cerebellum?
P. Roberts
Verified customer
Asked: 2016-07-01
Answer
According to the expression profile of cerebellum, RBFOX3 is highly expressed in cerebellum. So, it is likely that anti-NeuN Rabbit Monoclonal antibody M11954 will work for IF with cerebellum.
Boster Scientific Support
Answered: 2016-07-01
