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Product Info Summary
| SKU: | A13065 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RPC8 Antibody
SKU/Catalog Number
A13065
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPC8 Antibody (Catalog # A13065). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13065)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPC8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13065 is reactive to POLR3H in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
22918 MW
Background of Polr3h
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity.
Hu P., Mol. Cell. Biol. 22:8044-8055(2002).
Hirosawa M., DNA Res. 8:1-9(2001).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13065 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using RPC8 antibody A13065.
Protein Target Info & Infographic
Gene/Protein Information For POLR3H (Source: Uniprot.org, NCBI)
Gene Name
POLR3H
Full Name
DNA-directed RNA polymerase III subunit RPC8
Weight
22918 MW
Superfamily
eukaryotic RPB7/RPC8 RNA polymerase subunit family
Alternative Names
DNA-directed RNA polymerase III subunit RPC8;RNA polymerase III subunit C8;DNA-directed RNA polymerase III subunit H;RNA polymerase III subunit 22.9 kDa subunit;RPC22.9;POLR3H;KIAA1665, RPC8; Polr3h|5031409G22Rik, RP, RPC8|polymerase (RNA) III (DNA directed) polypeptide H|DNA-directed RNA polymerase III subunit RPC8|DNA-directed RNA polymerase III subunit H|RNA polymerase III subunit C7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3H, check out the POLR3H Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3H: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPC8 Antibody (A13065)
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Customer Q&As
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4 Customer Q&As for Anti-RPC8 Antibody
Question
Does A13065 anti-RPC8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Zhao
Verified customer
Asked: 2019-05-02
Answer
You can see on the product datasheet, A13065 anti-RPC8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-02
Question
Is there a BSA free version of anti-RPC8 antibody A13065 available?
J. Parker
Verified customer
Asked: 2018-04-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RPC8 antibody A13065 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-27
Question
My question regarding product A13065, anti-RPC8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13065 anti-RPC8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-26
Question
I have attached the WB image, lot number and protocol we used for brain pituitary using anti-RPC8 antibody A13065. Please let me know if you require anything else.
R. Roberts
Verified customer
Asked: 2014-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-09
