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Key=image_label, value=Western blot analysis of Nectin 2 expression in SW480 cell lysate.
Key=small_image_label, value=Western blot analysis of LSD2 / AOF1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate.
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Key=description, value=Boster Bio Anti-Nectin 2 Rabbit Monoclonal Antibody catalog # M08081-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key=short_description, value=Boster Bio Anti-Nectin 2 Rabbit Monoclonal Antibody catalog # M08081-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
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Key=contents, value=Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Key=background
Key=gene_name, value=NECTIN2
Key=synonyms, value=Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1;
Key=kit_components, value=-
Key=application_details, value=WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:100
FC 1:50
Key=subcellular_localization, value= Secreted. Released from the secretory granules upon mast cell activation. .
Key=tissue_specificity, value= Smooth muscle, and tissues containing significant amounts of smooth muscle.
Key=protein_function, value= Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). .
Key=application_notes, value=Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
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Key=immunogen, value=A synthesized peptide derived from human Nectin 2
Key=purification, value=Affinity-chromatography
Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
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Anti-Nectin 2 Rabbit Monoclonal Antibody

Nectin-2/CD112 antibody

Boster Bio Anti-Nectin 2 Rabbit Monoclonal Antibody catalog # M08081-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M08081-2
Size: 100 μl/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

Product Name

Anti-Nectin 2 Rabbit Monoclonal Antibody

View all Nectin-2/CD112 Antibodies

SKU/Catalog Number

M08081-2

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Nectin 2 Rabbit Monoclonal Antibody catalog # M08081-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nectin 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08081-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

20N15

Isotype

IgG

Immunogen

A synthesized peptide derived from human Nectin 2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08081-2 is reactive to NECTIN2 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

62-75 kDa

Calculated molecular weight

33170 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M08081-2 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:100
FC 1:50

Positive Control

WB: SW480 cell

Validation Images & Assay Conditions

Gene/Protein Information For NECTIN2 (Source: Uniprot.org, NCBI)

Gene Name

NECTIN2

Full Name

Nectin-2

Weight

33170 MW

Superfamily

nectin family

Alternative Names

Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1; NECTIN2 CD112, HVEB, PRR2, PVRL2, PVRR2 nectin cell adhesion molecule 2 nectin-2|herpesvirus entry protein B|poliovirus receptor-like 2|poliovirus receptor-related 2 (herpesvirus entry mediator B)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NECTIN2, check out the NECTIN2 Infographic

NECTIN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NECTIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M08081-2

100μL

$370
M08081-2-30ul

30μL sample

$99
M08081-2-carrier-free-1ml

Carrier Free

$2590

More conjugates/formats

Free Secondary Antibody

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M08081-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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