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Product Info Summary
| SKU: | M05867 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NDUFS3 Monoclonal Antibody
SKU/Catalog Number
M05867
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NDUFS3 Monoclonal Antibody catalog # M05867. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDUFS3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05867)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACIG-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NDUFS3 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05867 is reactive to NDUFS3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
150 kDa
Calculated molecular weight
113086 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05867 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of NDUFS3 expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NDUFS3 (Source: Uniprot.org, NCBI)
Gene Name
NDUFS3
Full Name
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
Weight
113086 MW
Superfamily
complex I 30 kDa subunit family
Alternative Names
Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein ;GM130 ;GM130 autoantigen ;Golgin-95 ;GOLGA2; NDUFS3 CI-30, MC1DN8 NADH:ubiquinone oxidoreductase core subunit S3 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial|CI-30kD|NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)|NADH dehydrogenase-ubiquinone 30 kDa subunit|NADH-ubiquinone oxidoreductase 30 kDa subunit|complex I 30kDa subunit|complex I-30kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFS3, check out the NDUFS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFS3 Monoclonal Antibody (M05867)
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No publications found for M05867
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Customer Q&As
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3 Customer Q&As for Anti-NDUFS3 Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for brain cajal-retzius cell using anti-NDUFS3 Monoclonal antibody M05867. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
My question regarding product M05867, anti-NDUFS3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05867 anti-NDUFS3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-NDUFS3 Monoclonal antibody M05867 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The anti-NDUFS3 Monoclonal antibody (M05867) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-05
