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Product Info Summary
| SKU: | PB9227 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband®
View all Maxi Potassium channel alpha Antibodies
SKU/Catalog Number
PB9227
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband® catalog # PB9227. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9227)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KCNMA1 recombinant protein (Position: K124-Q467). Human KCNMA1 shares 99% amino acid (aa) sequence identity with both mouse and rat KCNMA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9227 is reactive to KCNMA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110-138 kDa
Calculated molecular weight
137560 MW
Background of Maxi Potassium channel alpha
Calcium-activated potassium channel subunit alpha-1 also known as KCa1.1, or BK channel for short, is a voltage gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. This gene is located on 10q22.3. BK channels are activated (opened) by changes in membrane electrical potential and/or by increases in concentration of intracellular calcium ion (Ca2+). It is essential for the regulation of several key physiological processes including smooth muscle tone and neuronal excitability. BK channels also contribute to the behavioral effects of ethanol in the worm C. elegans under high concentrations (> 100 mM, or approximately 0.50% BAC).It remains to be determined if BK channels contribute to intoxication in humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9227 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
WB: human PC-3 whole cell, human U87 whole cell
IHC: mouse intestine tissue, rat intestine tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human U87 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNMA1 antigen affinity purified polyclonal antibody (Catalog # PB9227) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNMA1 at approximately 110-138 kDa. The expected band size for KCNMA1 is at 138KD.
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Figure 2. IHC analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). KCNMA1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KCNMA1 Antibody (PB9227) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). KCNMA1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KCNMA1 Antibody (PB9227) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). KCNMA1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KCNMA1 Antibody (PB9227) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KCNMA1 (Source: Uniprot.org, NCBI)
Gene Name
KCNMA1
Full Name
Calcium-activated potassium channel subunit alpha-1
Weight
137560 MW
Superfamily
potassium channel family
Alternative Names
Calcium-activated potassium channel subunit alpha-1;BK channel;BKCA alpha;Calcium-activated potassium channel, subfamily M subunit alpha-1;K (VCA)alpha;KCa1.1;Maxi K channel;MaxiK;Slo-alpha;Slo1;Slowpoke homolog;Slo homolog;hSlo;KCNMA1;KCNMA, SLO; KCNMA1 BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1 potassium calcium-activated channel subfamily M alpha 1 calcium-activated potassium channel subunit alpha-1|uncharacterized protein LOC3778|BK channel alpha subunit|BKCA alpha subunit|big potassium channel alpha subunit|calcium-activated potassium channel, subfamily M subunit alpha-1|k(VCA)alpha|maxi-K channel HSLO|potassium channel, calcium activated large conductance subfamily M alpha, member 1|potassium large conductance calcium-activated channel, subfamily M, alpha member 1|slo homolog|slowpoke homolog|stretch-activated Kca channel
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNMA1, check out the KCNMA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNMA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband® (PB9227)
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5 Customer Q&As for Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband®
Question
Will PB9227 anti-Maxi Potassium channel alpha/KCNMA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
You can see on the product datasheet, PB9227 anti-Maxi Potassium channel alpha/KCNMA1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-13
Question
We are currently using anti-Maxi Potassium channel alpha/KCNMA1 antibody PB9227 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
E. Yang
Verified customer
Asked: 2019-10-10
Answer
The anti-Maxi Potassium channel alpha/KCNMA1 antibody (PB9227) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
I have a question about product PB9227, anti-Maxi Potassium channel alpha/KCNMA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Wu
Verified customer
Asked: 2018-10-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9227 anti-Maxi Potassium channel alpha/KCNMA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-04
Question
you antibody to test anti-Maxi Potassium channel alpha/KCNMA1 antibody PB9227 on rat cerebellum for research purposes, then I may be interested in using anti-Maxi Potassium channel alpha/KCNMA1 antibody PB9227 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The products we sell, including anti-Maxi Potassium channel alpha/KCNMA1 antibody PB9227, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-03
Question
I see that the anti-Maxi Potassium channel alpha/KCNMA1 antibody PB9227 works with IHC, what is the protocol used to produce the result images on the product page?
T. Dhar
Verified customer
Asked: 2014-11-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-11-10
