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Product Info Summary
| SKU: | A05867 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NDUFS3 Antibody (C-term)
SKU/Catalog Number
A05867
Size
80 µl
Description
Boster Bio Anti-NDUFS3 Antibody (C-term) (Catalog # A05867). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NDUFS3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05867)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NDUFS3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 251-284 amino acids from the C-terminal region of human NDUFS3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05867 is reactive to NDUFS3 in Human, Mouse
Applications
A05867 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
30242 Da
Background of NDUFS3
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, HepG2, mouse NIH/3T3 cell line, human brain and mouse brain tissue lysate (from left to right), using NDUFS3 Antibody (C-term). A05867 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Western blot analysis of lysate from THP-1 cell line, using NDUFS3 Antibody (C-term) (Cat. #A05867). A05867 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Protein Target Info & Infographic
Gene/Protein Information For NDUFS3 (Source: Uniprot.org, NCBI)
Gene Name
NDUFS3
Full Name
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
Weight
30242 Da
Superfamily
complex I 30 kDa subunit family
Alternative Names
CI-30; CI-30kD; complex I 30kDa subunit; Complex I-30kD; EC 1.6.5.3; EC 1.6.99.3; EC 1.6.99.5; NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase); NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; NADH dehydrogenase-ubiquinone 30 kDa subunit; NADH-ubiquinone oxidoreductase 30 kDa subunit NDUFS3 CI-30, MC1DN8 NADH:ubiquinone oxidoreductase core subunit S3 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial|CI-30kD|NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)|NADH dehydrogenase-ubiquinone 30 kDa subunit|NADH-ubiquinone oxidoreductase 30 kDa subunit|complex I 30kDa subunit|complex I-30kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFS3, check out the NDUFS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFS3 Antibody (C-term) (A05867)
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