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Product Info Summary
| SKU: | A04072 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Pinch-1 LIMS1 Monoclonal Antibody
View all PINCH1/LIMS1 Antibodies
SKU/Catalog Number
A04072
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Pinch-1 LIMS1 Monoclonal Antibody catalog # A04072. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pinch-1 LIMS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04072)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Purified recombinant fragment of human Pinch-1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04072 is reactive to LIMS1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
37251 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04072 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IF, 1:200-1:1000
FC, 1:200-1:400
Validation Images & Assay Conditions
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Western Blot (WB) analysis using Pinch-1 Monoclonal antibody against A549 (1), Jurkat (2), and HeLa (3) cell lysate.
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Flow cytometric (FCM) analysis of HeLa cells using Pinch-1 Monoclonal antibody (blue) and negative control (red).
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Immunofluorescence (IF) analysis of HepG2 cells using Pinch-1 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA analysis of Pinch-1 antibody.
Protein Target Info & Infographic
Gene/Protein Information For LIMS1 (Source: Uniprot.org, NCBI)
Gene Name
LIMS1
Full Name
LIM and senescent cell antigen-like-containing domain protein 1
Weight
37251 MW
Alternative Names
LIM and senescent cell antigen-like-containing domain protein 1;Particularly interesting new Cys-His protein 1;PINCH-1;Renal carcinoma antigen NY-REN-48;LIMS1;PINCH, PINCH1; LIMS1 PINCH, PINCH-1, PINCH1 LIM zinc finger domain containing 1 LIM and senescent cell -like-containing domain protein 1|LIM and senescent cell -like domains 1|LIM-type zinc finger domains 1|particularly interesting new Cys-His protein 1|renal carcinoma NY-REN-48|senescent cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIMS1, check out the LIMS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIMS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pinch-1 LIMS1 Monoclonal Antibody (A04072)
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No publications found for A04072
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Pinch-1 LIMS1 Monoclonal Antibody
Question
Is this A04072 anti-Pinch-1 Monoclonal antibody reactive to the isotypes of LIMS1?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The immunogen of A04072 anti-Pinch-1 Monoclonal antibody is Purified recombinant fragment of human Pinch-1 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-29
Question
We want to test anti-Pinch-1 Monoclonal antibody A04072 on human testis for research purposes, then I may be interested in using anti-Pinch-1 Monoclonal antibody A04072 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The products we sell, including anti-Pinch-1 Monoclonal antibody A04072, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-19
Question
I see that the anti-Pinch-1 Monoclonal antibody A04072 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-17
Question
Is a blocking peptide available for product anti-Pinch-1 Monoclonal antibody (A04072)?
B. Taylor
Verified customer
Asked: 2019-10-03
Answer
We do provide the blocking peptide for product anti-Pinch-1 Monoclonal antibody (A04072). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-03
Question
Please see the WB image, lot number and protocol we used for testis using anti-Pinch-1 Monoclonal antibody A04072. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
We are currently using anti-Pinch-1 Monoclonal antibody A04072 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-Pinch-1 Monoclonal antibody (A04072) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
Do you have a BSA free version of anti-Pinch-1 Monoclonal antibody A04072 available?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Pinch-1 Monoclonal antibody A04072 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-25
Question
My question regarding product A04072, anti-Pinch-1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Moore
Verified customer
Asked: 2018-06-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04072 anti-Pinch-1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-12
Question
Will A04072 anti-Pinch-1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
As indicated on the product datasheet, A04072 anti-Pinch-1 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Pinch-1 Monoclonal antibody A04072. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-19
Question
Does anti-Pinch-1 Monoclonal antibody A04072 work on zebrafish IHC with endometrium?
A. Krishna
Verified customer
Asked: 2016-03-08
Answer
Our lab technicians have not tested anti-Pinch-1 Monoclonal antibody A04072 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Pinch-1 Monoclonal antibody A04072 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish endometrium in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-08
Question
I was wanting to use your anti-Pinch-1 Monoclonal antibody for IHC for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
G. Evans
Verified customer
Asked: 2015-01-27
Answer
You can see on the product datasheet, A04072 anti-Pinch-1 Monoclonal antibody has been validated for Flow Cytometry, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-27
Question
Would anti-Pinch-1 Monoclonal antibody A04072 work for IHC with testis?
A. Baker
Verified customer
Asked: 2014-06-19
Answer
According to the expression profile of testis, LIMS1 is highly expressed in testis. So, it is likely that anti-Pinch-1 Monoclonal antibody A04072 will work for IHC with testis.
Boster Scientific Support
Answered: 2014-06-19
