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Product Info Summary
| SKU: | A07393-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-NAT8L Antibody Picoband®
SKU/Catalog Number
A07393-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAT8L Antibody Picoband® catalog # A07393-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NAT8L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07393-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NAT8L, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07393-2 is reactive to NAT8L in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
108320 MW
Background of Nat8l
N-acetyltransferase (NAT) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to arylamines, arylhydroxylamines and arylhydrazines. It is mapped to 4p16.3. This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07393-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
"Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
"
Positive Control
WB: human HEK293 whole cell, human A549 whole cell, human U-87MG whole cell, human Hela whole cell
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry analysis of U20S cells using anti-NAT8L antibody (A07393-2).
Overlay histogram showing U20S cells stained with A07393-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAT8L Antibody (A07393-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 1. Western blot analysis of NAT8L using anti-NAT8L antibody (A07393-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAT8L antigen affinity purified polyclonal antibody (Catalog # A07393-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for NAT8L at approximately 33-40KD. The expected band size for NAT8L is at 33KD.
Protein Target Info & Infographic
Gene/Protein Information For NAT8L (Source: Uniprot.org, NCBI)
Gene Name
NAT8L
Full Name
N-acetylaspartate synthetase
Weight
108320 MW
Superfamily
camello family
Alternative Names
N-acetylaspartate synthetase; NAA synthetase; Camello-like protein 3; N-acetyltransferase 8-like protein; NAT8L; CML3 Nat8l|1110038O08Rik, Sh, Shati|N-acetyltransferase 8-like|N-acetylaspartate synthetase|N-acetyltransferase 8-like protein|NAA synthase|NAA synthetase|aspartate N-acetyltransferase|protein Shati
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAT8L, check out the NAT8L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAT8L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAT8L Antibody Picoband® (A07393-2)
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Customer Q&As
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1 Customer Q&As for Anti-NAT8L Antibody Picoband®
Question
We are currently using anti-NAT8L antibody A07393-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
N. Li
Verified customer
Asked: 2013-06-19
Answer
The anti-NAT8L antibody (A07393-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-19
