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Product Info Summary
| SKU: | A05100-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NAP1L1 Antibody Picoband®
SKU/Catalog Number
A05100-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAP1L1 Antibody Picoband® catalog # A05100-1. Tested in IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NAP1L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05100-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NAP1L1, which shares 95.7% amino acid (aa) sequence identity with mouse and rat NAP1L1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05100-1 is reactive to NAP1L1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
75991 MW
Background of NAP1L1
Nucleosome assembly protein 1-like 1 is a protein that in humans is encoded by the NAP1L1 gene. This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms; however, not all have been fully described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05100-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse NIH/3T3 whole cell
ICC/IF: U2OS cell
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NAP1L1 using anti-NAP1L1 antibody (A05100-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP1L1 antigen affinity purified polyclonal antibody (Catalog # A05100-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAP1L1 at approximately 55 kDa. The expected band size for NAP1L1 is at 45 kDa.
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Figure 2. IF analysis of NAP1L1 using anti-NAP1L1 antibody (A05100-1).
NAP1L1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NAP1L1 Antibody (A05100-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-NAP1L1 antibody (A05100-1).
Overlay histogram showing PC-3 cells stained with A05100-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAP1L1 Antibody (A05100-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NAP1L1 (Source: Uniprot.org, NCBI)
Gene Name
NAP1L1
Full Name
Nucleosome assembly protein 1-like 1
Weight
75991 MW
Superfamily
nucleosome assembly protein (NAP) family
Alternative Names
Sentrin-specific protease 6; SUMO-1-specific protease 1; Sentrin/SUMO-specific protease SENP6; SENP6; KIAA0797; SSP1; SUSP1; FKSG6 NAP1L1 NAP1, NAP1L, NRP nucleosome assembly protein 1 like 1 nucleosome assembly protein 1-like 1|HSP22-like protein interacting protein|NAP-1-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAP1L1, check out the NAP1L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAP1L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAP1L1 Antibody Picoband® (A05100-1)
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