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Product Info Summary
| SKU: | M01577-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody
View all N-Cadherin Antibodies
SKU/Catalog Number
M01577-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody catalog # M01577-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01577-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human N Cadherin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01577-1 is reactive to CDH2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
140 kDa
Calculated molecular weight
99809 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01577-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human 293T whole cell, human Hela whole cell, human A549 whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
IHC: Rat testis tissue, Rat stomach tissue, Human colon cancer tissue, Human prostate cancer tissue, Mouse skin tissue, human colon tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:100 dilution.
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Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (M01577-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # M01577-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140 kDa. The expected band size for CDH2 is at 100 kDa.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon, using N Cadherin Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDH2 (Source: Uniprot.org, NCBI)
Gene Name
CDH2
Full Name
Cadherin-2
Weight
99809 MW
Alternative Names
Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD325;CDH2;CDHN, NCAD; CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH2, check out the CDH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody (M01577-1)
Hello CJ!
M01577-1 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
M01577-1 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200
Jia X, Wang H,Li Z,Yan J,Guo Y,Zhao W,Gao L,Wang B,Jia Y.HER4 promotes the progression of colorectal cancer by promoting epithelial‑mesenchymal transition.Mol Med Rep.2020 Apr;21(4):1779-1788.doi:10. 3892/mmr.2020.10974.Epub 2020 Feb 4.PMID:32319604;PMCID
Species: Human
M01577-1 usage in article: APP:WB, SAMPLE:HCT116 CELL, DILUTION:1:200
Expression of N%u2011cadherin proteins in myocardial hypertrophy in rats
Expression of N-cadherin in myocardial tissues during the development of a rat heart
Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta%u2013mediated epithelial%u2013mesenchymal transition
Expression of N-cadherin proteins in myocardial hypertrophy in rats
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
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