Product Info Summary
SKU: | PB9112 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-MUC1 Antibody Picoband™
SKU/Catalog Number
PB9112
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MUC1 Antibody Picoband™ catalog # PB9112. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9112)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse MUC1 recombinant protein (Position: S474-L630). Mouse MUC1 shares 87% and amino acid (aa) sequence identity with human MUC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9112 is reactive to MUC1 in Mouse, Rat
Applications
PB9112 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
120 kDa, 150 kDa, 280 kDa
Calculated molecular weight
64539 MW
Background of MUC1
Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Validation Images & Assay Conditions
![pb9112 1 pb9112 1](https://www.bosterbio.com/media/catalog/product/p/b/pb9112-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of MUC1 using anti-MUC1 antibody (PB9112).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC1 antigen affinity purified polyclonal antibody (Catalog # PB9112) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC1 at approximately 120,150,280KD. The expected band size for MUC1 is at 120-400KD.
![pb9112 3 IHC anti muc1 picoband antibody pb9112 3 IHC anti muc1 picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb9112-3-IHC-anti-muc1-picoband-antibody.jpg)
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Figure 2. IHC analysis of MUC1 using anti-MUC1 antibody (PB9112).
MUC1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUC1 Antibody (PB9112) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pb9112 4 IHC anti muc1 picoband antibody pb9112 4 IHC anti muc1 picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb9112-4-IHC-anti-muc1-picoband-antibody.jpg)
Click image to see more details
Figure 3. IHC analysis of MUC1 using anti-MUC1 antibody (PB9112).
MUC1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUC1 Antibody (PB9112) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pb9112 4_1 pb9112 4_1](https://www.bosterbio.com/media/catalog/product/p/b/pb9112-4_1.jpg)
Click image to see more details
Figure 4. IHC analysis of MUC1 using anti-MUC1 antibody (PB9112).
MUC1 was detected in frozen section of mouse lung tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUC1 Antibody (PB9112) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MUC1 (Source: Uniprot.org, NCBI)
Gene Name
MUC1
Full Name
Mucin-1
Weight
64539 MW
Alternative Names
Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 antigen|breast carcinoma-associated antigen DF3|cancer antigen 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC1, check out the MUC1 Infographic
![MUC1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC1 Antibody Picoband™ (PB9112)
Hello CJ!
PB9112 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Globose, cystic olfactory ensheathing cell tumor: A case report and literature review
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.
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Customer Q&As
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1 Customer Q&As for Anti-MUC1 Antibody Picoband™
Question
I would like using your anti-MUC1 antibody for r-mmu-913709; o-linked glycosylation of mucins studies. Has this antibody been tested with western blotting on rh35 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
I appreciate your inquiry. This PB9112 anti-MUC1 antibody is tested on rh35 whole cell lysate, rat kidney, mouse lung. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-16