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Product Info Summary
| SKU: | EK1232 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TFF1/PS2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1232
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TFF1/Ps2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TFF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TFF1/PS2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1232)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: E25-F84
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1232 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1232 is reactive to TFF1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1232 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TFF1/pS2
TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1232-CAP | Anti-Human TFF1 Pre-coated 96-well strip microplate | 1 |
| EK1232-ST | Human TFF1 Standard | 2 vials, 1 ng/tube |
| EK1232-DA | Human TFF1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.052 | 0.220 | 0.321 | 0.523 | 0.866 | 1.253 | 1.722 | 2.013 |
Data Example Images
Click image to see more details
Human TFF1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TFF1/pS2, Dilution ratio of 1:1, concentration in serum and plasma is around 50 pg/ml..
Some articles we found to cite concentrations of TFF1/pS2 in samples: 21699780 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TFF1/PS2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 177 | 433 | 38 | 193 | 420 |
| Standard deviation | 1.96 | 9.91 | 29.87 | 2.16 | 11 | 29.4 |
| CV (%) | 5.6% | 5.6% | 6.9% | 5.7% | 5.7% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TFF1/pS2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 37 | 38 | 40 | 37 | 1.8 | 4.8% |
| Sample 2 | 177 | 154 | 156 | 174 | 165 | 10.32 | 6.2% |
| Sample 3 | 433 | 394 | 416 | 409 | 413 | 14.01 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For TFF1 (Source: Uniprot.Org, NCBI)
Gene Name
TFF1
Full Name
Trefoil factor 1
Weight
9150 MW
Alternative Names
Trefoil factor 1;Breast cancer estrogen-inducible protein;PNR-2;Polypeptide P1.A;hP1.A;Protein pS2;TFF1;BCEI, PS2; TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2 trefoil factor 1 trefoil factor 1|breast cancer estrogen-inducible protein|breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2|polypeptide P1.A|protein pS2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TFF1, check out the TFF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TFF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TFF1/PS2 ELISA Kit PicoKine® (EK1232)
Hello CJ!
EK1232 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Novel urinary protein biomarker panel for early diagnosis of gastric cancer
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Customer Reviews
Have you used Human TFF1/PS2 ELISA Kit PicoKine®?
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1 Reviews For Human TFF1/PS2 ELISA Kit PicoKine®
0
Competitively priced and worked--Lu Yang, UCSD, CMMW, GRADUATE STUDENT
Excellent
Source: Biocompare.com
| Applications | Protein level screen |
|---|---|
| Sample | Cell culture media from MCF7 cells |
| Detection | -- |
"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked as advertised."
Lu Yang
Verified customer
Submitted 2016-02-17
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Human TFF1/PS2 ELISA Kit PicoKine®
Question
Can you confirm if the volume of Biotinylated antibody and Avidin-Biotin-Peroxidase Complex has been changed from 130ul to 100ul for the Human TFF1/Ps2 ELISA Kit PicoKine™ (EK1232)?
Verified customer
Asked: 2021-01-28
Answer
The volume of Biotinylated antibody and Avidin-Biotin-Peroxidase Complex have been changed from 130ul to 100ul. All future lots contains 100 ul for those two components. Our lab takes reasonable loss into account and provide more than 100ul and they are sufficient for users to finish the assay.
Boster Scientific Support
Answered: 2021-01-28
Question
Will the Human TFF1/Ps2 ELISA Kit PicoKine work for all sample types?
Verified customer
Asked: 2019-11-05
Answer
Yes, we guarantee the kit to work on the sample types that we have tested for and if target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-11-05
Question
What is the recommended dilution for serum samples using EK1232?
Verified customer
Asked: 2019-11-05
Answer
The recommended dilution for serum samples using the Human TFF1/Ps2 ELISA Kit PicoKine™ (EK1232) is 1:1.
Boster Scientific Support
Answered: 2019-11-05
Question
Can the EK1232 kit be used to detect TFF1 in Human urine samples?
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not validated the Human TFF1/Ps2 ELISA Kit PicoKine EK1232 with urine samples, so we cannot guarantee that it would work. In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-10-23
Question
Q: Can TFF1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-03
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
H. Singh
Verified customer
Asked: 2019-04-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-04-29
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Puri
Verified customer
Asked: 2018-05-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-05-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Krishna
Verified customer
Asked: 2017-02-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-02-23
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TFF1 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2015-09-17
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TFF1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2015-09-17
