Product Info Summary
SKU: | M00187-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MUC1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00187-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MUC1 Rabbit Monoclonal Antibody catalog # M00187-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00187-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FHA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MUC1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00187-1 is reactive to MUC1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
122102 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00187-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:20
Positive Control
IHC: human ovarian cancer tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of MUC1 expression in T47 D cell lysate.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using MUC1 Antibody.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For MUC1 (Source: Uniprot.org, NCBI)
Gene Name
MUC1
Full Name
Mucin-1
Weight
122102 MW
Alternative Names
Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM; MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 |breast carcinoma-associated DF3|cancer 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC1, check out the MUC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC1 Rabbit Monoclonal Antibody (M00187-1)
Hello CJ!
M00187-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Globose, cystic olfactory ensheathing cell tumor: A case report and literature review
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-MUC1 Rabbit Monoclonal Antibody
Question
Will M00187-1 anti-MUC1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Patel
Verified customer
Asked: 2020-05-08
Answer
You can see on the product datasheet, M00187-1 anti-MUC1 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
Is a blocking peptide available for product anti-MUC1 Rabbit Monoclonal antibody (M00187-1)?
R. Li
Verified customer
Asked: 2020-04-09
Answer
We do provide the blocking peptide for product anti-MUC1 Rabbit Monoclonal antibody (M00187-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-09
Question
I see that the anti-MUC1 Rabbit Monoclonal antibody M00187-1 works with IHC, what is the protocol used to produce the result images on the product page?
R. Johnson
Verified customer
Asked: 2020-03-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-26
Question
Do you have a BSA free version of anti-MUC1 Rabbit Monoclonal antibody M00187-1 available?
M. Krishna
Verified customer
Asked: 2020-01-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUC1 Rabbit Monoclonal antibody M00187-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-02
Question
I have attached the WB image, lot number and protocol we used for carcinoma using anti-MUC1 Rabbit Monoclonal antibody M00187-1. Please let me know if you require anything else.
C. Baker
Verified customer
Asked: 2019-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
Does anti-MUC1 Rabbit Monoclonal antibody M00187-1 work for IHC with carcinoma?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of carcinoma, MUC1 is highly expressed in carcinoma. So, it is likely that anti-MUC1 Rabbit Monoclonal antibody M00187-1 will work for IHC with carcinoma.
Boster Scientific Support
Answered: 2019-07-04
Question
Our lab used your anti-MUC1 Rabbit Monoclonal antibody for WB on mammary carcinoma in a previous project. I am using human, and We want to use the antibody for IHC next. Our lab want to know about examining mammary carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
I have checked the website and datasheets of our anti-MUC1 Rabbit Monoclonal antibody and it appears that M00187-1 has been tested on human in both WB and IHC. Thus M00187-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-06
Question
We are currently using anti-MUC1 Rabbit Monoclonal antibody M00187-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
The anti-MUC1 Rabbit Monoclonal antibody (M00187-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-23
Question
Can you check to be sure the correct WB image is upload for this product? https://www.bosterbio.com/anti-muc1-rabbit-monoclonal-antibody-m00187-1-boster.html?options=cart The size is ~100 kDa low.
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
According to Uniprot https://www.uniprot.org/uniprot/P15941, there are 17 isoforms of MUC1. For some of them, the Mass (Da) are around 17-30kDa. Immunogen sequence of M00187-1 is CGSSLSYTNPAVAAASANL. The band between 20 and 25kDa is the only one detected by them.
Boster Scientific Support
Answered: 2017-12-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for carcinoma using anti-MUC1 Rabbit Monoclonal antibody M00187-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-09
Question
I am looking for to test anti-MUC1 Rabbit Monoclonal antibody M00187-1 on human carcinoma for research purposes, then I may be interested in using anti-MUC1 Rabbit Monoclonal antibody M00187-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
The products we sell, including anti-MUC1 Rabbit Monoclonal antibody M00187-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-12
Question
Can you help my question with product M00187-1, anti-MUC1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00187-1 anti-MUC1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-22
Question
I was wanting to use your anti-MUC1 Rabbit Monoclonal antibody for IHC for human carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human carcinoma identification?
J. Parker
Verified customer
Asked: 2016-03-21
Answer
It shows on the product datasheet, M00187-1 anti-MUC1 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-21
Question
Our lab were well pleased with the WB result of your anti-MUC1 Rabbit Monoclonal antibody. However we have observed positive staining in lung isoform 9: secreted. using this antibody. Is that expected? Could you tell me where is MUC1 supposed to be expressed?
R. Mangal
Verified customer
Asked: 2015-11-05
Answer
According to literature, lung does express MUC1. Generally MUC1 expresses in apical cell membrane, isoform 5: secreted., isoform y: secreted., isoform 9: secreted., mucin-1 subunit beta: cell membrane. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722
Boster Scientific Support
Answered: 2015-11-05
Question
Does anti-MUC1 Rabbit Monoclonal antibody M00187-1 work on primate IP with carcinoma?
P. Banerjee
Verified customer
Asked: 2014-10-03
Answer
Our lab technicians have not validated anti-MUC1 Rabbit Monoclonal antibody M00187-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MUC1 Rabbit Monoclonal antibody M00187-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate carcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-03
Question
We have observed staining in human body of stomach. Are there any suggestions? Is anti-MUC1 Rabbit Monoclonal antibody supposed to stain body of stomach positively?
C. Baker
Verified customer
Asked: 2013-08-08
Answer
From what I have seen in literature body of stomach does express MUC1. From what I have seen in Uniprot.org, MUC1 is expressed in body of stomach, pancreatic carcinoma, mammary carcinoma, carcinoma, cervix carcinoma, thyroid, lung, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722
Boster Scientific Support
Answered: 2013-08-08
Question
Is this M00187-1 anti-MUC1 Rabbit Monoclonal antibody reactive to the isotypes of MUC1?
T. Johnson
Verified customer
Asked: 2013-02-14
Answer
The immunogen of M00187-1 anti-MUC1 Rabbit Monoclonal antibody is A synthesized peptide derived from human MUC1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-02-14