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Product Info Summary
| SKU: | PA1360 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TGF alpha/TGFA Antibody Picoband®
SKU/Catalog Number
PA1360
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TGF alpha/TGFA Antibody catalog # PA1360. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGF alpha/TGFA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1360)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TGF alpha, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1360 is reactive to TGFA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
6 kDa
Calculated molecular weight
17006 MW
Background of TGF-alpha
Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGFalpha stimulates neural cell proliferation in the adult injured brain. TGFalpha was cited in the 2001 NIH Stem Cell report to the U.S. Congress as promising evidence for the ability of adult stem cells to restore function in neurodegenerative disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1360 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-TGF alpha antibody, PA1360, Western blotting
All lanes: Anti TGF alpha (PA1360) at 0.5ug/ml
Lane 1: Recombinant Human TGF a Protein 10ng
Lane 2: Recombinant Human TGF a Protein 5ng
Lane 3: Recombinant Human TGF a Protein 2.5ng
Predicted bind size: 6KD
Observed bind size: 6KD
Protein Target Info & Infographic
Gene/Protein Information For TGFA (Source: Uniprot.org, NCBI)
Gene Name
TGFA
Full Name
Protransforming growth factor alpha
Weight
17006 MW
Alternative Names
Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA; TGFA TFGA transforming growth factor alpha protransforming growth factor alpha|TGF-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFA, check out the TGFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF alpha/TGFA Antibody Picoband® (PA1360)
Hello CJ!
PA1360 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ruan Gp, Xu F, Li Za, Zhu Gx, Pang Rq, Wang Jx, Cai Xm, He J, Yao X, Ruan Gh, Xu Xm, Pan Xh. Plos One. 2013 Dec 18;8(12):E83507. Doi: 10.1371/Journal.Pone.0083507. Ecollection 2013. Induced Autologous Stem Cell Transplantation For Treatment Of Rab...
Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TGF alpha/TGFA Antibody Picoband®
Question
I see that the anti-TGF alpha/TGFA antibody PA1360 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-24
Question
Is a blocking peptide available for product anti-TGF alpha/TGFA antibody (PA1360)?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We do provide the blocking peptide for product anti-TGF alpha/TGFA antibody (PA1360). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-20
Question
Does anti-TGF alpha/TGFA antibody PA1360 work for WB with thalamus?
A. Parker
Verified customer
Asked: 2017-10-05
Answer
According to the expression profile of thalamus, TGFA is highly expressed in thalamus. So, it is likely that anti-TGF alpha/TGFA antibody PA1360 will work for WB with thalamus.
Boster Scientific Support
Answered: 2017-10-05
Question
We are currently using anti-TGF alpha/TGFA antibody PA1360 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
T. Mangal
Verified customer
Asked: 2014-05-02
Answer
The anti-TGF alpha/TGFA antibody (PA1360) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-02
Question
See below the WB image, lot number and protocol we used for thalamus using anti-TGF alpha/TGFA antibody PA1360. Please let me know if you require anything else.
T. Lewis
Verified customer
Asked: 2013-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-01
