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Product Info Summary
| SKU: | A04180-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MT-ND4 Antibody Picoband®
SKU/Catalog Number
A04180-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MT-ND4 Antibody Picoband® catalog # A04180-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MT-ND4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04180-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04180-1 is reactive to MT-ND4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
51.581kDa
Background of MT-ND4
NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04180-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human THP-1 whole cell, human Caco-2 whole cell, human U2OS whole cell, human A549 whole cell,
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MT-ND4 using anti-MT-ND4 antibody (A04180-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates
Lane 2: human HepG2 whole cell lysates
Lane 3: human THP-1 whole cell lysates
Lane 4: human Caco-2 whole cell lysates
Lane 5: human U2OS whole cell lysates
Lane 6: human A549 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND4 antigen affinity purified polyclonal antibody (Catalog # A04180-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-ND4 at approximately 36KD. The expected band size for MT-ND4 is at 52KD.
Protein Target Info & Infographic
Gene/Protein Information For MT-ND4 (Source: Uniprot.org, NCBI)
Gene Name
MT-ND4
Full Name
NADH-ubiquinone oxidoreductase chain 4
Weight
51.581kDa
Superfamily
complex I subunit 4 family
Alternative Names
NADH-ubiquinone oxidoreductase chain 4; NADH dehydrogenase subunit 4; MT-ND4; MTND4; NADH4; ND4;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MT-ND4, check out the MT-ND4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MT-ND4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MT-ND4 Antibody Picoband® (A04180-1)
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Customer Q&As
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1 Customer Q&As for Anti-MT-ND4 Antibody Picoband®
Question
We are currently using anti-MT-ND4 antibody A04180-1 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The anti-MT-ND4 antibody (A04180-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-20
