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Product Info Summary
| SKU: | M13360-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | IP, WB |
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Product info
Product Name
Anti-Ms Lime1 Purified Monoclonal Antibody
SKU/Catalog Number
M13360-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms Lime1 Purified Monoclonal Antibody (Catalog# M13360-1). Tested in IP, WB application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms Lime1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13360-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
mLIME-05
Isotype
Mouse IgG2a
Immunogen
Recombinant protein corresponding to amino acids 86-202 of mouse LIME. Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M13360-1 is reactive to LIME1 in Mouse
Applications
M13360-1 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
29551 MW
Background of LIME
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunoprecipitation: Positive control: murine splenic T cells.
Western blotting: Reducing conditions, recommended dilution: 1-5 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Ms Lime1 Purified Monoclonal Antibody M13360-1
Western blotting analysis of murine thymocyte lysate (1% SDS); non-reduced sampleProtein Target Info & Infographic
Gene/Protein Information For LIME1 (Source: Uniprot.org, NCBI)
Gene Name
LIME1
Full Name
Lck-interacting transmembrane adapter 1
Weight
29551 MW
Alternative Names
dJ583P15.4; FLJ20406; Lck interacting transmembrane adaptor 1; Lck-interacting membrane protein; Lck-interacting molecule; lck-interacting transmembrane adapter 1; LIMELP8067 LIME1 LIME, dJ583P15.4 Lck interacting transmembrane adaptor 1 lck-interacting transmembrane adapter 1|lck-interacting membrane protein|lck-interacting molecule
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIME1, check out the LIME1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ms Lime1 Purified Monoclonal Antibody (M13360-1)
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