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Product Info Summary
| SKU: | A00197 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TLR3 Antibody Picoband®
SKU/Catalog Number
A00197
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TLR3 Antibody Picoband® catalog # A00197. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00197)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TLR3 recombinant protein (Position: F502-D648).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00197 is reactive to TLR3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
103.829kDa
Background of TLR3
TLR3 (Toll-like receptor 3) also known as CD283 (cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene is mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-κB and the production of type I interferons. It may thus play a role in host defense against viruses. TLR3 recognizes double-stranded RNA, a form of genetic information carried by some viruses such as retroviruses. Upon recognition, TLR3 induces the activation of NF-κB to increase production of type I interferons which signal other cells to increase their antiviral defenses. Double-stranded RNA is also recognised by the cytoplasmic receptors RIG-I and MDA-5. TLR3 displays a protective role in mouse models of atherosclerosis.[4] In addition, TLR3 activators show effects on human vascular cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00197 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Jurkat whole cell, mouse Raw2647 whole cell, human K562 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TLR3 using anti-TLR3 antibody (A00197).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: mouse Raw264.7 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR3 antigen affinity purified polyclonal antibody (Catalog # A00197) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR3 at approximately 120 kDa. The expected band size for TLR3 is at 104 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TLR3 (Source: Uniprot.org, NCBI)
Gene Name
TLR3
Full Name
Toll-like receptor 3
Weight
103.829kDa
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 3; CD283; TLR3 TLR3 CD283, IIAE2 toll like receptor 3 toll-like receptor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR3, check out the TLR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TLR3 Antibody Picoband® (A00197)
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Customer Q&As
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16 Customer Q&As for Anti-TLR3 Antibody Picoband®
Question
Is a blocking peptide available for product anti-TLR3 antibody (A00197)?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We do provide the blocking peptide for product anti-TLR3 antibody (A00197). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-TLR3 antibody A00197. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
Is this A00197 anti-TLR3 antibody reactive to the isotypes of TLR3?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The immunogen of A00197 anti-TLR3 antibody is E. coli-derived human TLR3 recombinant protein (Position: F502-D648). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-TLR3 antibody A00197 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
D. Miller
Verified customer
Asked: 2019-09-10
Answer
The anti-TLR3 antibody (A00197) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-10
Question
I was wanting to use your anti-TLR3 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
As indicated on the product datasheet, A00197 anti-TLR3 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
I am interested in to test anti-TLR3 antibody A00197 on rat liver for research purposes, then I may be interested in using anti-TLR3 antibody A00197 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Thomas
Verified customer
Asked: 2019-08-02
Answer
The products we sell, including anti-TLR3 antibody A00197, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-02
Question
My team were happy with the WB result of your anti-TLR3 antibody. However we have observed positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is TLR3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
According to literature, liver does express TLR3. Generally TLR3 expresses in endoplasmic reticulum membrane. Regarding which tissues have TLR3 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 12539043
Neuron, Pubmed ID: 17085778
Placenta, Pubmed ID: 15489334
Testis, and Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-11-28
Question
We want using your anti-TLR3 antibody for positive regulation of type iii interferon production studies. Has this antibody been tested with western blotting on lung tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
We appreciate your inquiry. This A00197 anti-TLR3 antibody is validated on mouse pancreas tissue, lung tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-11
Question
We have observed staining in mouse placenta. Do you have any suggestions? Is anti-TLR3 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
From literature placenta does express TLR3. From Uniprot.org, TLR3 is expressed in placenta, neuron, testis thymus, lung, liver, among other tissues. Regarding which tissues have TLR3 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 12539043
Neuron, Pubmed ID: 17085778
Placenta, Pubmed ID: 15489334
Testis, and Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-23
Question
We ordered your anti-TLR3 antibody for ELISA on testis thymus last year. I am using rat, and We intend to use the antibody for WB next. I was wanting to use examining testis thymus as well as neuron in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
I took a look at the website and datasheets of our anti-TLR3 antibody and it appears that A00197 has been validated on rat in both ELISA and WB. Thus A00197 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-22
Question
I see that the anti-TLR3 antibody A00197 works with WB, what is the protocol used to produce the result images on the product page?
G. Baker
Verified customer
Asked: 2017-09-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-08
Question
Can you help my question with product A00197, anti-TLR3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Wu
Verified customer
Asked: 2017-05-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00197 anti-TLR3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-25
Question
Does anti-TLR3 antibody A00197 work for WB with liver?
B. Anderson
Verified customer
Asked: 2016-09-22
Answer
According to the expression profile of liver, TLR3 is highly expressed in liver. So, it is likely that anti-TLR3 antibody A00197 will work for WB with liver.
Boster Scientific Support
Answered: 2016-09-22
Question
Here is the WB image, lot number and protocol we used for liver using anti-TLR3 antibody A00197. Please let me know if you require anything else.
V. Anderson
Verified customer
Asked: 2015-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-17
Question
Is there a BSA free version of anti-TLR3 antibody A00197 available?
J. Carter
Verified customer
Asked: 2015-02-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-TLR3 antibody A00197 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-17
Question
Does A00197 anti-TLR3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Edwards
Verified customer
Asked: 2013-03-06
Answer
As indicated on the product datasheet, A00197 anti-TLR3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-06
