Click image to see more details
Product Info Summary
| SKU: | A04485 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband®
SKU/Catalog Number
A04485
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband® catalog # A04485. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04485)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IRF9 recombinant protein (Position: M1-K110). Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04485 is reactive to IRF9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
43696 MW
Background of IRF9
Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. It is mapped to 14q1. IRF9 is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. In addition, IRF9 is an interferon-dependent, positive-acting transcription factor that is cytoplasmically activated, possibly through direct interaction with the interferon receptor. It associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04485 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IRF9 using anti-IRF9 antibody (A04485).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: JURKAT cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF9 antigen affinity purified polyclonal antibody (Catalog # A04485) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF9 at approximately 44KD. The expected band size for IRF9 is at 44KD.
Protein Target Info & Infographic
Gene/Protein Information For IRF9 (Source: Uniprot.org, NCBI)
Gene Name
IRF9
Full Name
Interferon regulatory factor 9
Weight
43696 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 9;IRF-9;IFN-alpha-responsive transcription factor subunit;ISGF3 p48 subunit;Interferon-stimulated gene factor 3 gamma;ISGF-3 gamma;Transcriptional regulator ISGF3 subunit gamma;IRF9;ISGF3G; IRF9 IRF-9, ISGF3, ISGF3G, p48 interferon regulatory factor 9 interferon regulatory factor 9|IFN-alpha-responsive transcription factor subunit|ISGF-3 gamma|ISGF3 p48 subunit|interferon-stimulated gene factor 3 gamma|interferon-stimulated transcription factor 3, gamma (48kD)|interferon-stimulated transcription factor 3, gamma 48kDa|transcriptional regulator ISGF3 subunit gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF9, check out the IRF9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband® (A04485)
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Customer Q&As
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13 Customer Q&As for Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for ovary using anti-Interferon regulatory factor 9/IRF9 antibody A04485. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
Is there a BSA free version of anti-Interferon regulatory factor 9/IRF9 antibody A04485 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Interferon regulatory factor 9/IRF9 antibody A04485 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-Interferon regulatory factor 9/IRF9 antibody A04485. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
Is a blocking peptide available for product anti-Interferon regulatory factor 9/IRF9 antibody (A04485)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do provide the blocking peptide for product anti-Interferon regulatory factor 9/IRF9 antibody (A04485). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-05
Question
I was wanting to use your anti-Interferon regulatory factor 9/IRF9 antibody for WB for human ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovary identification?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can see on the product datasheet, A04485 anti-Interferon regulatory factor 9/IRF9 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-03
Question
I see that the anti-Interferon regulatory factor 9/IRF9 antibody A04485 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-11
Question
We want to test anti-Interferon regulatory factor 9/IRF9 antibody A04485 on human ovary for research purposes, then I may be interested in using anti-Interferon regulatory factor 9/IRF9 antibody A04485 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The products we sell, including anti-Interferon regulatory factor 9/IRF9 antibody A04485, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-30
Question
Would anti-Interferon regulatory factor 9/IRF9 antibody A04485 work on goat WB with ovary?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Our lab technicians have not tested anti-Interferon regulatory factor 9/IRF9 antibody A04485 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Interferon regulatory factor 9/IRF9 antibody A04485 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Can you help my question with product A04485, anti-Interferon regulatory factor 9/IRF9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04485 anti-Interferon regulatory factor 9/IRF9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-15
Question
Is this A04485 anti-Interferon regulatory factor 9/IRF9 antibody reactive to the isotypes of IRF9?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
The immunogen of A04485 anti-Interferon regulatory factor 9/IRF9 antibody is E. coli-derived human IRF9 recombinant protein (Position: M1-K110). Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-17
Question
Will A04485 anti-Interferon regulatory factor 9/IRF9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Jackson
Verified customer
Asked: 2017-08-25
Answer
As indicated on the product datasheet, A04485 anti-Interferon regulatory factor 9/IRF9 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-25
Question
Does anti-Interferon regulatory factor 9/IRF9 antibody A04485 work for WB with ovary?
J. Jha
Verified customer
Asked: 2017-04-27
Answer
According to the expression profile of ovary, IRF9 is highly expressed in ovary. So, it is likely that anti-Interferon regulatory factor 9/IRF9 antibody A04485 will work for WB with ovary.
Boster Scientific Support
Answered: 2017-04-27
Question
We are currently using anti-Interferon regulatory factor 9/IRF9 antibody A04485 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
V. Jones
Verified customer
Asked: 2015-04-20
Answer
The anti-Interferon regulatory factor 9/IRF9 antibody (A04485) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-20
