Click image to see more details
Product Info Summary
| SKU: | EK1500 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human EDIL3 ELISA Kit PicoKine®
View all EDIL3/DEL1 ELISA kits
SKU/Catalog Number
EK1500
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EDIL3 ELISA Kit PicoKine® (96 Tests). Quantitate Human EDIL3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EDIL3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1500)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D24-E480
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1500 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1500 is reactive to EDIL3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1500 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EDIL3/DEL1
EDIL3, also known as DEL1, is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. It is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis. The EDIL3 gene is mapped to 5q14. Human endothelial cells bind to Del1 together with the alpha-V (ITGAV)/beta-3 (ITGB3) integrin receptor. It is confirmed that adhesion of endothelial cells to Del1 is integrin mediated and that Del1 binds ITGAV/ITGB3. Functional analysis showed that both Del1 and vitronectin (VTN) stimulate endothelial cell migration. Immunoprecipitation and immunoblot analyses revealed that adhesion of endothelial cells to Del1 induces tyrosine phosphorylation of PTK2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1500-CAP | Anti-Human EDIL3 Pre-coated 96-well strip microplate | 1 |
| EK1500-ST | Human EDIL3 Standard | 2 vials, 10 ng/tube |
| EK1500-DA | Human EDIL3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.175 | 0.221 | 0.236 | 0.310 | 0.415 | 0.734 | 1.252 | 1.962 |
Data Example Images
Click image to see more details
Human EDIL3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EDIL3/DEL1, Dilution ratio of 1:1, concentration in serum and plasma is around 150 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EDIL3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 56 | 181 | 804 | 59 | 203 | 803 |
| Standard deviation | 1.52 | 7.17 | 30.55 | 1.4 | 5.22 | 60.47 |
| CV(%) | 2.7% | 4% | 3.8% | 2.4% | 2.6% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of EDIL3/DEL1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 36 | 57 | 60 | 52 | 3.62 | 6.9% |
| Sample 2 | 181 | 196 | 202 | 205 | 196 | 6.25 | 3.2% |
| Sample 3 | 804 | 970 | 736 | 552 | 766 | 35.37 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For EDIL3 (Source: Uniprot.Org, NCBI)
Gene Name
EDIL3
Full Name
EGF-like repeat and discoidin I-like domain-containing protein 3
Weight
53765 MW
Alternative Names
EGF-like repeat and discoidin I-like domain-containing protein 3;Developmentally-regulated endothelial cell locus 1 protein;Integrin-binding protein DEL1;EDIL3;DEL1; EDIL3 DEL1 EGF like repeats and discoidin domains 3 EGF-like repeat and discoidin I-like domain-containing protein 3|EGF-like repeats and discoidin I-like domains 3|developmental endothelial locus-1|developmentally-regulated endothelial cell locus 1 protein|integrin-binding protein DEL1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EDIL3, check out the EDIL3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EDIL3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EDIL3 ELISA Kit PicoKine® (EK1500)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human EDIL3 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human EDIL3 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human EDIL3 ELISA Kit PicoKine®
Question
Can EK1500 be used for testing whole blood samples?
Verified customer
Asked: 2021-01-27
Answer
Unfortunately, our lab hasn't tested the Human EDIL3 ELISA Kit PicoKine™ (EK1500) on whole blood samples. Please run a pilot test to see if the kit is able to work on whole blood samples.
Boster Scientific Support
Answered: 2021-01-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-31
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-08-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-08-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Martinez
Verified customer
Asked: 2020-02-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-29
Question
Q: if the enzyme conjugated EDIL3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the EDIL3 antigen?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-10-31
Question
Q: how can I thaw whole blood sample for EDIL3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-07-15
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EDIL3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-07-15
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis EDIL3 using the EK1500 Human EDIL3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-04-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2015-04-17
