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Product Info Summary
| SKU: | A13607 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MRPL18 Antibody
SKU/Catalog Number
A13607
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL18 Antibody (Catalog # A13607). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13607)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPL18.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13607 is reactive to MRPL18 in Human
Reconstitution
Calculated molecular weight
20577 MW
Background of MRPL18
Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA.
Wiemann S., Genome Res. 11:422-435(2001).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13607 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using MRPL18 antibody A13607.
Protein Target Info & Infographic
Gene/Protein Information For MRPL18 (Source: Uniprot.org, NCBI)
Gene Name
MRPL18
Full Name
39S ribosomal protein L18, mitochondrial
Weight
20577 MW
Superfamily
universal ribosomal protein uL18 family
Alternative Names
39S ribosomal protein L18, mitochondrial;L18mt;MRP-L18;MRPL18;HSPC071; MRPL18 HSPC071, L18mt, MRP-L18 mitochondrial ribosomal protein L18 39S ribosomal protein L18, mitochondrial|mitochondrial large ribosomal subunit protein uL18m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL18, check out the MRPL18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL18 Antibody (A13607)
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No publications found for A13607
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-MRPL18 Antibody
Question
I see that the anti-MRPL18 antibody A13607 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-21
Question
I have a question about product A13607, anti-MRPL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13607 anti-MRPL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-01
Question
Is this A13607 anti-MRPL18 antibody reactive to the isotypes of MRPL18?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The immunogen of A13607 anti-MRPL18 antibody is Synthesized peptide derived from internal of human MRPL18. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-20
Question
We are currently using anti-MRPL18 antibody A13607 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
O. Moore
Verified customer
Asked: 2019-09-13
Answer
The anti-MRPL18 antibody (A13607) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
Question
Would A13607 anti-MRPL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
It shows on the product datasheet, A13607 anti-MRPL18 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-26
Question
Here is the WB image, lot number and protocol we used for umbilical cord blood using anti-MRPL18 antibody A13607. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-15
