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Product Info Summary
| SKU: | A00974-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MRP2/ABCC2 Antibody Picoband®
SKU/Catalog Number
A00974-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MRP2/ABCC2 Antibody Picoband® catalog # A00974-1. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP2/ABCC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00974-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MRP2/ABCC2, which shares 77.1% and 71.4% amino acid (aa) sequence identity with mouse and rat MRP2/ABCC2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00974-1 is reactive to ABCC2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170-250 kDa
Calculated molecular weight
174.207kDa
Background of MRP2
Multidrug resistance-associated protein 2 (MRP2), also called canalicular multispecific organic anion transporter 1 (cMOAT) or ATP-binding cassette sub-family C member 2 (ABCC2), is a protein that in humans is encoded by the ABCC2 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00974-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human HepG2 whole cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP2/ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP2/ABCC2 at approximately 170-250 kDa. The expected band size for MRP2/ABCC2 is at 174 kDa.
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Figure 2. IHC analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1).
MRP2/ABCC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRP2/ABCC2 Antibody (A00974-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1).
MRP2/ABCC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRP2/ABCC2 Antibody (A00974-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ABCC2 (Source: Uniprot.org, NCBI)
Gene Name
ABCC2
Full Name
Canalicular multispecific organic anion transporter 1
Weight
174.207kDa
Superfamily
ABC transporter superfamily
Alternative Names
Canalicular multispecific organic anion transporter 1; ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2; ABCC2; CMOAT; CMOAT1; CMRP; MRP2 ABCC2 ABC30, CMOAT, DJS, MRP2, cMRP ATP binding cassette subfamily C member 2 canalicular multispecific organic anion transporter 1|ATP-binding cassette, sub-family C (CFTR/MRP), member 2|canalicular multidrug resistance protein|multidrug resistance-associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCC2, check out the ABCC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP2/ABCC2 Antibody Picoband® (A00974-1)
Hello CJ!
A00974-1 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cisplatin-induced CCL5 secretion from CAFs promotes cisplatin-resistance in ovarian cancer via regulation of the STAT3 and PI3K/Akt signaling pathways
Lentivirus-mediated RNAi silencing targeting ABCC2 increasing the sensitivity of a human nasopharyngeal carcinoma cell line against cisplatin
The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice
Association of ABCC2 and CDDP-Resistance in Two Sublines Resistant to CDDP Derived from a Human Nasopharyngeal Carcinoma Cell Line
Songsong Wang,Xiao Xiao,Ao Li,Peng Li,"The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 5566078,9 pages,2021.https://doi.org/10.1155/2021/5566078
Species: Mouse
A00974-1 usage in article: APP:WB, SAMPLE:HEPATORENAL TISSUE, DILUTION:NA
Huang J,Wang Q,Chen M,Bi Y,Shi H,Zhou K. Effects of psoralen on hepatic bile acid transporters in rats. Hum Exp Toxicol.2020 Dec 15:960327120979346.doi:10.1177/0960327120979346. Epub ahead of print.PMID:33317360.
Species: Rat
A00974-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500
Association of ABCC2 and CDDP-resistance in two sublines resistant to CDDP derived from a human nasopharyngeal carcinoma cell line
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Customer Q&As
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1 Customer Q&As for Anti-MRP2/ABCC2 Antibody Picoband®
Question
We are currently using anti-MRP2/ABCC2 antibody A00974-1 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The anti-MRP2/ABCC2 antibody (A00974-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
