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Product Info Summary
| SKU: | PA1670 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband®
SKU/Catalog Number
PA1670
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sodium/bile acid cotransporter SLC10A1 Antibody catalog # PA1670. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1670)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1670 is reactive to SLC10A1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
38119 MW
Background of SLC10A1
Na+-taurocholate cotransporting polypeptide (NTCP), also known as SLC10A1 (Solute carrier family 10, member 1), is the major bile acid uptake system in human hepatocytes. NTCP and the ileal transporter ASBT (apical sodium-dependent bile acid transporter) are two sodium-dependent transporters critical for the enterohepatic circulation of bile acids. The hASBT gene is known to be activated by the glucocorticoid receptor (GR). Ho RH et al. indicates functionally important polymorphisms in NTCP exist and that the like lihood of being carriers of such polymorphisms is dependent on ethnicity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1670 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Positive Control
WB: human liver tissue, rat liver tissue, rat RH35 whole cell, rat kidney tissue, rat brain tissue, mouse liver tissue, mouse HEPA1-6 whole cell, mouse kidney tissue, mouse brain tissue
IHC: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human liver tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (Catalog # PA1670) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50 kDa. The expected band size for SLC10A1 is at 38 kDa.
Click image to see more details
Figure 2. Western blot analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat RH35 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse HEPA1-6 whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (Catalog # PA1670) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50 kDa. The expected band size for SLC10A1 is at 38 kDa.
Click image to see more details
Figure 3. IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670).
SLC10A1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670).
SLC10A1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SLC10A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC10A1
Full Name
Sodium/bile acid cotransporter
Weight
38119 MW
Superfamily
bile acid:sodium symporter (BASS) (TC 2.A.28) family
Alternative Names
Sodium/bile acid cotransporter;Cell growth-inhibiting gene 29 protein;Na (+)/bile acid cotransporter;Na (+)/taurocholate transport protein;Sodium/taurocholate cotransporting polypeptide;Solute carrier family 10 member 1;SLC10A1;NTCP;GIG29; SLC10A1 NTCP solute carrier family 10 member 1 sodium/bile acid cotransporter|Na(+)/bile acid cotransporter|Na(+)/taurocholate transport protein|Na/taurocholate cotransporting polypeptide|cell growth-inhibiting gene 29 protein|growth-inhibiting protein 29|sodium/taurocholate cotransporter|sodium/taurocholate cotransporting polypeptide|solute carrier family 10 (sodium/bile acid cotransporter family), member 1|solute carrier family 10 (sodium/bile acid cotransporter), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC10A1, check out the SLC10A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC10A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband® (PA1670)
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No publications found for PA1670
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Customer Q&As
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8 Customer Q&As for Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband®
Question
What is the dilution dilution ration for the primary antibody in PA1670?
Verified customer
Asked: 2022-04-29
Answer
The dilution ration for the primary antibody in the Anti-Sodium/Bile Acid Cotransporter SLC10A1 Antibody (PA1670) is 1:2000.
Boster Scientific Support
Answered: 2022-05-18
Question
Is a blocking peptide available for product anti-SLC10A1 antibody (PA1670)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We do provide the blocking peptide for product anti-SLC10A1 antibody (PA1670). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-16
Question
Does anti-SLC10A1 antibody PA1670 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
According to the expression profile of liver, SLC10A1 is highly expressed in liver. So, it is likely that anti-SLC10A1 antibody PA1670 will work for WB with liver.
Boster Scientific Support
Answered: 2019-05-31
Question
See attached the WB image, lot number and protocol we used for liver using anti-SLC10A1 antibody PA1670. Please let me know if you require anything else.
L. Banerjee
Verified customer
Asked: 2019-02-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-15
Question
Is this PA1670 anti-SLC10A1 antibody reactive to the isotypes of SLC10A1?
T. Patel
Verified customer
Asked: 2019-02-01
Answer
The immunogen of PA1670 anti-SLC10A1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1(141-154aa YSRGIYDGDLKDKV), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-01
Question
Does PA1670 anti-SLC10A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Johnson
Verified customer
Asked: 2018-02-27
Answer
It shows on the product datasheet, PA1670 anti-SLC10A1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-27
Question
Can you help my question with product PA1670, anti-SLC10A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Kulkarni
Verified customer
Asked: 2017-12-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1670 anti-SLC10A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-26
Question
I was wanting to use your anti-SLC10A1 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
A. Zhang
Verified customer
Asked: 2016-02-24
Answer
You can see on the product datasheet, PA1670 anti-SLC10A1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-02-24
