Anti-(Mouse) Cldn6 Antibody (C-term)

Product Name

Anti-(Mouse) Cldn6 Antibody (C-term)

View all Claudin-6 Antibodies

SKU/Catalog Number

M08182-1

Size

80 µl

Description

Boster Bio Anti-(Mouse) Cldn6 Antibody (C-term) (Catalog # M08182-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-(Mouse) Cldn6 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08182-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This Mouse Cldn6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 186-229 amino acids from the C-terminal region of mouse Cldn6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08182-1 is reactive to CLDN6 in Mouse

Applications

M08182-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

23388 Da

Background of Claudin-6

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
FC: 1:25

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term). M08182-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Click image to see more details

Overlay histogram showing colon26 cells stained with M08182-1 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08182-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For CLDN6 (Source: Uniprot.org, NCBI)

Alternative Names

claudin 6; Claudin6; Claudin-6; CLDN6; Skullin 2; skullin CLDN6 claudin 6 claudin-6|skullin

For more info on CLDN6, check out the CLDN6 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CLDN6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the CLDN6 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis