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Product Info Summary
| SKU: | M08182-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-(Mouse) Cldn6 Antibody (C-term)
SKU/Catalog Number
M08182-1
Size
80 µl
Description
Boster Bio Anti-(Mouse) Cldn6 Antibody (C-term) (Catalog # M08182-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Cldn6 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08182-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Cldn6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 186-229 amino acids from the C-terminal region of mouse Cldn6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08182-1 is reactive to Cldn6 in Mouse
Reconstitution
Calculated molecular weight
23388 Da
Background of Claudin-6
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08182-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term). M08182-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Overlay histogram showing colon26 cells stained with M08182-1 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08182-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For Cldn6 (Source: Uniprot.org, NCBI)
Gene Name
Cldn6
Full Name
Claudin-6
Weight
23388 Da
Superfamily
claudin family
Alternative Names
Claudin-6, Skullin, Cldn6 CLDN6 claudin 6 claudin-6|skullin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cldn6, check out the Cldn6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cldn6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-(Mouse) Cldn6 Antibody (C-term) (M08182-1)
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