Click image to see more details
Product Info Summary
| SKU: | P08182 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Phospho Claudin 6 (Tyr219) Antibody
SKU/Catalog Number
P08182
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Claudin 6 (Tyr219) Antibody (Catalog# P08182). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Claudin 6 (Tyr219) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P08182)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Claudin 6 around the phosphorylation site of Tyr219. Immunogen sequence location: 171-220
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P08182 is reactive to CLDN6 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
23292 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P08182 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using Claudin 6 (Phospho-Tyr219) Antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CLDN6 (Source: Uniprot.org, NCBI)
Gene Name
CLDN6
Full Name
Claudin-6
Weight
23292 MW
Superfamily
claudin family
Alternative Names
CLDN6;Claudin-6;Skullin CLDN6 claudin 6 claudin-6|skullin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN6, check out the CLDN6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho Claudin 6 (Tyr219) Antibody (P08182)
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No publications found for P08182
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho Claudin 6 (Tyr219) Antibody
Question
Does anti-Phospho Claudin 6 (Tyr219) antibody P08182 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to the expression profile of placenta, CLDN6 is highly expressed in placenta. So, it is likely that anti-Phospho Claudin 6 (Tyr219) antibody P08182 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-03-03
Question
We are currently using anti-Phospho Claudin 6 (Tyr219) antibody P08182 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-Phospho Claudin 6 (Tyr219) antibody (P08182) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
Can you help my question with product P08182, anti-Phospho Claudin 6 (Tyr219) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P08182 anti-Phospho Claudin 6 (Tyr219) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-20
Question
Our lab want to know about using your anti-Phospho Claudin 6 (Tyr219) antibody for tight junction interactions studies. Has this antibody been tested with western blotting on raw264.7 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
I appreciate your inquiry. This P08182 anti-Phospho Claudin 6 (Tyr219) antibody is validated on raw264.7 cells. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-18
Question
Here is the WB image, lot number and protocol we used for placenta using anti-Phospho Claudin 6 (Tyr219) antibody P08182. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-21
Question
Is a blocking peptide available for product anti-Phospho Claudin 6 (Tyr219) antibody (P08182)?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
We do provide the blocking peptide for product anti-Phospho Claudin 6 (Tyr219) antibody (P08182). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Phospho Claudin 6 (Tyr219) antibody P08182. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-15
Question
We have seen staining in mouse female gonad. Do you have any suggestions? Is anti-Phospho Claudin 6 (Tyr219) antibody supposed to stain female gonad positively?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
From what I have seen in literature female gonad does express CLDN6. From what I have seen in Uniprot.org, CLDN6 is expressed in female gonad, placenta, among other tissues. Regarding which tissues have CLDN6 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-12
Question
I was wanting to use your anti-Phospho Claudin 6 (Tyr219) antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
You can see on the product datasheet, P08182 anti-Phospho Claudin 6 (Tyr219) antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-03
Question
Does P08182 anti-Phospho Claudin 6 (Tyr219) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Jones
Verified customer
Asked: 2017-06-05
Answer
As indicated on the product datasheet, P08182 anti-Phospho Claudin 6 (Tyr219) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-05
Question
My colleagues were happy with the WB result of your anti-Phospho Claudin 6 (Tyr219) antibody. However we have seen positive staining in placenta cell junction using this antibody. Is that expected? Could you tell me where is CLDN6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
From literature, placenta does express CLDN6. Generally CLDN6 expresses in cell junction, tight junction. Regarding which tissues have CLDN6 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-16
Question
Is there a BSA free version of anti-Phospho Claudin 6 (Tyr219) antibody P08182 available?
J. Banerjee
Verified customer
Asked: 2016-08-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho Claudin 6 (Tyr219) antibody P08182 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-08-24
Question
you antibody to test anti-Phospho Claudin 6 (Tyr219) antibody P08182 on mouse placenta for research purposes, then I may be interested in using anti-Phospho Claudin 6 (Tyr219) antibody P08182 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Bhatt
Verified customer
Asked: 2015-03-04
Answer
The products we sell, including anti-Phospho Claudin 6 (Tyr219) antibody P08182, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-03-04
Question
I see that the anti-Phospho Claudin 6 (Tyr219) antibody P08182 works with WB, what is the protocol used to produce the result images on the product page?
Z. Moore
Verified customer
Asked: 2013-09-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-20
Question
Is this P08182 anti-Phospho Claudin 6 (Tyr219) antibody reactive to the isotypes of CLDN6?
W. Zhao
Verified customer
Asked: 2013-03-25
Answer
The immunogen of P08182 anti-Phospho Claudin 6 (Tyr219) antibody is A peptide derived from human Claudin 6 around the phosphorylation site of Tyr219. Immunogen sequence location: 171-220. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-25
