Product Info Summary
SKU: | PA1380 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Stromelysin-2 MMP10 Antibody Picoband®
SKU/Catalog Number
PA1380
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Stromelysin-2 MMP10 Antibody catalog # PA1380. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Stromelysin-2 MMP10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1380)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1380 is reactive to MMP10 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
54151 MW
Background of MMP-10
Stromelysin-2 also known as matrix metalloproteinase-10 (MMP-10) or transin-2 is an enzyme that in humans is encoded by the MMP10 gene.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1380 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MMP10 antibody, PA1380, Western blotting
Lane 1: Recombinant Human MMP-10 Protein 10ng
Lane 2: Recombinant Human MMP-10 Protein 5ng
Lane 3: Recombinant Human MMP-10 Protein 2.5ng
Lane 4: HELA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For MMP10 (Source: Uniprot.org, NCBI)
Gene Name
MMP10
Full Name
Stromelysin-2
Weight
54151 MW
Superfamily
peptidase M10A family
Alternative Names
Stromelysin-2;SL-2;3.4.24.22;Matrix metalloproteinase-10;MMP-10;Transin-2;MMP10;STMY2; MMP10 SL-2, STMY2 matrix metallopeptidase 10 stromelysin-2|MMP-10|matrix metalloprotease 10|matrix metalloproteinase 10 (stromelysin 2)|matrix metalloproteinase-10|transin 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP10, check out the MMP10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Stromelysin-2 MMP10 Antibody Picoband® (PA1380)
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Customer Q&As
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5 Customer Q&As for Anti-Stromelysin-2 MMP10 Antibody Picoband®
Question
Will anti-MMP10 antibody PA1380 work on canine WB with esophagus?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not tested anti-MMP10 antibody PA1380 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MMP10 antibody PA1380 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine esophagus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
I have a question about product PA1380, anti-MMP10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1380 anti-MMP10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-01
Question
Do you have a BSA free version of anti-MMP10 antibody PA1380 available?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP10 antibody PA1380 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-18
Question
I have attached the WB image, lot number and protocol we used for coronary artery using anti-MMP10 antibody PA1380. Please let me know if you require anything else.
B. Johnson
Verified customer
Asked: 2018-07-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-27
Question
Would anti-MMP10 antibody PA1380 work for WB with coronary artery?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
According to the expression profile of coronary artery, MMP10 is highly expressed in coronary artery. So, it is likely that anti-MMP10 antibody PA1380 will work for WB with coronary artery.
Boster Scientific Support
Answered: 2017-07-03