Anti-MMP-15 Antibody

MMP-15/MT2-MMP antibody

Boster Bio Anti-MMP-15 Antibody (Catalog # A06396). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A06396
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-MMP-15 Antibody

View all MMP-15/MT2-MMP Antibodies

SKU/Catalog Number

A06396

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MMP-15 Antibody (Catalog # A06396). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP-15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06396)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MMP-15.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06396 is reactive to MMP15 in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

75807 MW

Background of MMP-15/MT2-MMP

Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.

Reimar Abraham, J. Biol. Chem., Oct 2005; 280: 34123 - 34132.
Henrik Toft-Hansen, J. Immunol., Oct 2004; 173: 5209 - 5218.
Lucie E. Jones, Clin. Cancer Res., Apr 2004; 10: 2832 - 2845.
Yrj? T Konttinen, Ann Rheum Dis, Nov 1999; 58: 691 - 697.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06396 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For MMP15 (Source: Uniprot.org, NCBI)

Gene Name

MMP15

Full Name

Matrix metalloproteinase-15

Weight

75807 MW

Superfamily

peptidase M10A family

Alternative Names

Matrix metalloproteinase-15;MMP-15;3.4.24.-;Membrane-type matrix metalloproteinase 2;MT-MMP 2;MTMMP2;Membrane-type-2 matrix metalloproteinase;MT2-MMP;MT2MMP;SMCP-2;MMP15; MMP15 MMP-15, MT2-MMP, MT2MMP, MTMMP2, SMCP-2 matrix metallopeptidase 15 matrix metalloproteinase-15|MT-MMP 2|matrix metallopeptidase 15 (membrane-inserted)|matrix metalloproteinase 15 (membrane-inserted)|membrane-type matrix metalloproteinase 2|membrane-type-2 matrix metalloproteinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MMP15, check out the MMP15 Infographic

MMP15 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MMP15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-MMP-15 Antibody

Question

Does A06396 anti-MMP-15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Anderson

Verified customer

Asked: 2020-01-16

Answer

You can see on the product datasheet, A06396 anti-MMP-15 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-16

Question

I have attached the WB image, lot number and protocol we used for liver using anti-MMP-15 antibody A06396. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-09

Question

We are currently using anti-MMP-15 antibody A06396 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?

E. Dhar

Verified customer

Asked: 2019-11-25

Answer

The anti-MMP-15 antibody (A06396) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-25

Question

I am looking for to test anti-MMP-15 antibody A06396 on mouse liver for research purposes, then I may be interested in using anti-MMP-15 antibody A06396 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Zhao

Verified customer

Asked: 2015-02-09

Answer

The products we sell, including anti-MMP-15 antibody A06396, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-02-09

Order DetailsPrice
A06396-100uL

100uL

$360

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