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Product Info Summary
| SKU: | A06396 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MMP-15 Antibody
View all MMP-15/MT2-MMP Antibodies
SKU/Catalog Number
A06396
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MMP-15 Antibody (Catalog # A06396). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP-15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06396)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MMP-15.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06396 is reactive to MMP15 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
75807 MW
Background of MMP-15/MT2-MMP
Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Reimar Abraham, J. Biol. Chem., Oct 2005; 280: 34123 - 34132.
Henrik Toft-Hansen, J. Immunol., Oct 2004; 173: 5209 - 5218.
Lucie E. Jones, Clin. Cancer Res., Apr 2004; 10: 2832 - 2845.
Yrj? T Konttinen, Ann Rheum Dis, Nov 1999; 58: 691 - 697.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06396 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from MDA-MB-435 cells, using MMP-15 antibody A06396.
Protein Target Info & Infographic
Gene/Protein Information For MMP15 (Source: Uniprot.org, NCBI)
Gene Name
MMP15
Full Name
Matrix metalloproteinase-15
Weight
75807 MW
Superfamily
peptidase M10A family
Alternative Names
Matrix metalloproteinase-15;MMP-15;3.4.24.-;Membrane-type matrix metalloproteinase 2;MT-MMP 2;MTMMP2;Membrane-type-2 matrix metalloproteinase;MT2-MMP;MT2MMP;SMCP-2;MMP15; MMP15 MMP-15, MT2-MMP, MT2MMP, MTMMP2, SMCP-2 matrix metallopeptidase 15 matrix metalloproteinase-15|MT-MMP 2|matrix metallopeptidase 15 (membrane-inserted)|matrix metalloproteinase 15 (membrane-inserted)|membrane-type matrix metalloproteinase 2|membrane-type-2 matrix metalloproteinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP15, check out the MMP15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP-15 Antibody (A06396)
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Customer Q&As
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4 Customer Q&As for Anti-MMP-15 Antibody
Question
Does A06396 anti-MMP-15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Anderson
Verified customer
Asked: 2020-01-16
Answer
You can see on the product datasheet, A06396 anti-MMP-15 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
I have attached the WB image, lot number and protocol we used for liver using anti-MMP-15 antibody A06396. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-MMP-15 antibody A06396 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
E. Dhar
Verified customer
Asked: 2019-11-25
Answer
The anti-MMP-15 antibody (A06396) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
I am looking for to test anti-MMP-15 antibody A06396 on mouse liver for research purposes, then I may be interested in using anti-MMP-15 antibody A06396 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Zhao
Verified customer
Asked: 2015-02-09
Answer
The products we sell, including anti-MMP-15 antibody A06396, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-09
