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Product Info Summary
| SKU: | A00319 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-DNMT3B Antibody
SKU/Catalog Number
A00319
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DNMT3B Antibody (Catalog # A00319). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNMT3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00319)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human DNMT3B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00319 is reactive to DNMT3B in Human
Reconstitution
Calculated molecular weight
95751 MW
Background of DNMT3B
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing By similarity. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.
Deloukas P., Nature 414:865-871(2001).
Li H., J. Biol. Chem. 281:19489-19500(2006).
Wijmenga C., Hum. Mutat. 16:509-517(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00319 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using DNMT3B antibody A00319.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using DNMT3B antibody A00319.
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Western blot analysis of extracts from HuvEc cells (Lane 2), using DNMT3B antiobdy A00319. The lane on the left is treated with synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using DNMT3B antibody A00319.
Protein Target Info & Infographic
Gene/Protein Information For DNMT3B (Source: Uniprot.org, NCBI)
Gene Name
DNMT3B
Full Name
DNA (cytosine-5)-methyltransferase 3B
Weight
95751 MW
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
DNA (cytosine-5)-methyltransferase 3B;Dnmt3b;2.1.1.37;DNA methyltransferase HsaIIIB;DNA MTase HsaIIIB;M.HsaIIIB;DNMT3B; DNMT3B ICF, ICF1, M.HsaIIIB DNA methyltransferase 3 beta DNA (cytosine-5)-methyltransferase 3B|DNA (cytosine-5-)-methyltransferase 3 beta|DNA MTase HsaIIIB|DNA cytosine-5--methyltransferase 3 beta|DNA methyltransferase HsaIIIB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNMT3B, check out the DNMT3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNMT3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNMT3B Antibody (A00319)
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