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Product Info Summary
| SKU: | A12261 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MLIP Antibody
SKU/Catalog Number
A12261
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MLIP Antibody (Catalog # A12261). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
MLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MLIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12261)
Host
Rabbit
Contents
MLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MLIP antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human MLIP. The immunogen is located within the last 50 amino acids of MLIP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of MLIP are known to exist.
Reactive Species
A12261 is reactive to MLIP in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
50429 MW
Background of Mlip
The muscle-enriched A-type lamin-interacting protein (MLIP) is ubiquitously expressed, although most predominantly in heart, skeletal and smooth muscle, and interacts directly and co-localizes with lamin A and C in the nuclear envelope. MLIP also co-localizes with promyelocytic leukemia (PML) bodies within the nucleus. Decreasing lamin A/C expression with siRNA results in the upregulation and mislocalization of MLIP, suggesting that MLIP localization to the nuclear envelope is dependent on the lamin proteins. It has been suggested that MLIP may function as a tethering protein linking LMNA to factors within the nuclear envelope of within PML bodies
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12261 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MLIP antibody can be used for detection of MLIP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MLIP in 293 cell lysate with MLIP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of MLIP in human heart tissue with MLIP antibody at 5 μg/mL.
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Immunofluorescence of MLIP in human heart tissue with MLIP antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MLIP (Source: Uniprot.org, NCBI)
Gene Name
MLIP
Full Name
Muscular LMNA-interacting protein
Weight
50429 MW
Alternative Names
C6orf142, C6orf142, Muscular LMNA-interacting protein, Muscular-enriched A-type laminin-interacting protein Mlip|2310046A06Rik, AI931782, C, CIP|muscular LMNA-interacting protein|muscular LMNA-interacting protein|cardiac Isl1-interacting protein|muscular-enriched A-type laminin-interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MLIP, check out the MLIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MLIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MLIP Antibody (A12261)
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