Product Info Summary
SKU: | M00269-1 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Dog, Human |
Host: | Mouse |
Application: | IHC |
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Product info
Product Name
Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody
SKU/Catalog Number
M00269-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody (Catalog # M00269-1). Tested in IHC applications. This antibody reacts with Human, Dog.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00269-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: MITF/915
Isotype
IgG1, kappa
Immunogen
Recombinant full-length human MiTF protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00269-1 is reactive to MITF in Dog, Human
Applications
M00269-1 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58795 MW
Background of MITF
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This monoclonal antibody recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-MITF Mouse Monoclonal Antibody (MITF/915).
Protein Target Info & Infographic
Gene/Protein Information For MITF (Source: Uniprot.org, NCBI)
Gene Name
MITF
Full Name
Microphthalmia-associated transcription factor
Weight
58795 MW
Superfamily
MiT/TFE family
Alternative Names
bHLHe32; bHLHe32MI; Class E basic helix-loop-helix protein 32; MI; microphthalmia-associated transcription factor; MITF; Waardenburg syndrome, type 2A; WS2A MITF CMM8, COMMAD, MI, WS2, WS2A, bHLHe32 melanocyte inducing transcription factor microphthalmia-associated transcription factor|class E basic helix-loop-helix protein 32|melanogenesis associated transcription factor|microphtalmia-associated transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MITF, check out the MITF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MITF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody (M00269-1)
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